Partitioning of the linear chromosome during sporulation of Streptomyces coelicolor A3(2) involves an oriC-linked parAB locus

被引:97
作者
Kim, HJ
Calcutt, MJ
Schmidt, FJ
Chater, KF
机构
[1] John Innes Inst, Dept Genet, Norwich NR4 7UH, Norfolk, England
[2] Univ Missouri, Dept Mol Microbiol & Immunol, Columbia, MO USA
[3] Univ Missouri, Canc Res Ctr, Columbia, MO USA
[4] Univ Missouri, Dept Biochem, Columbia, MO USA
关键词
D O I
10.1128/JB.182.5.1313-1320.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Candidate partitioning genes (parA and parB) for the linear chromosome of Streptomyces coelicolor were identified by DNA sequencing in a series of seven genes located between rnpA and trxA near the chromosomal replication origin. The most likely translation start point of parB overlapped the parA stop codon, suggestive of coregulation, and transcription analysis suggested that the two genes formed an operon. Deletion of part of parB had no effect on the growth or appearance of colonies but caused a deficiency in DNA partitioning during the multiple septation events involved in converting aerial hyphae into long chains of spores. At least 13% of spore compartments failed to inherit the normal DNA allocation. The same phenotype was obtained with a deletion removing a segment of DNA from both parA and parB. Reinforcing the idea of a special role for the par locus during sporulation, the stronger of two parAB promoters was greatly upregulated at about the time when sporulation septation was maximal in colonies. Three copies of a 14-bp inverted repeat (GTTTCACGT GAAAC) were found in or near the parAB genes, and at least 12 more identical copies were identified within 100 kb of oriC from the growing genome sequence database. Only one perfect copy of the 14-bp sequence was present in approximately 5 Mb of sequence available from the rest of the genome. The 14-bp sequence was similar to sequences identified as binding sites for Spo0J, a ParB homologue from Bacillus subtilis believed to be important for DNA partitioning (D. C.-H. Lin and A. D. Grossman, Cell 92:675-685, 1998). One of these sites encompassed the transcription start point of the stronger parA promoter.
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页码:1313 / 1320
页数:8
相关论文
共 47 条
[21]  
Hopwood D.A., 1985, GENETIC MANIPULATION
[22]   SPO0J IS REQUIRED FOR NORMAL CHROMOSOME SEGREGATION AS WELL AS THE INITIATION OF SPORULATION IN BACILLUS-SUBTILIS [J].
IRETON, K ;
GUNTHER, NW ;
GROSSMAN, AD .
JOURNAL OF BACTERIOLOGY, 1994, 176 (17) :5320-5329
[23]   Structural elements of the Streptomyces oriC region and their interactions with the DnaA protein [J].
Jakimowicz, D ;
Majka, J ;
Messer, W ;
Speck, C ;
Fernandez, M ;
Martin, MC ;
Sanchez, J ;
Schauwecker, F ;
Keller, U ;
Schrempf, H ;
Zakrzewska-Czerwinska, J .
MICROBIOLOGY-SGM, 1998, 144 :1281-1290
[24]   Partitioning of plasmid R1. The ParM protein exhibits ATPase activity and interacts with the centromere-like ParR-parC complex [J].
Jensen, RB ;
Gerdes, K .
JOURNAL OF MOLECULAR BIOLOGY, 1997, 269 (04) :505-513
[25]   Mechanism of DNA segregation in prokaryotes: ParM partitioning protein of plasmid R1 co-localizes with its replicon during the cell cycle [J].
Jensen, RB ;
Gerdes, K .
EMBO JOURNAL, 1999, 18 (14) :4076-4084
[26]  
KIESER T, 1991, METHOD ENZYMOL, V204, P430
[27]   Direct evidence for active segregation of oriC regions of the Bacillus subtilis chromosome and co-localization with the SpoOJ partitioning protein [J].
Lewis, PJ ;
Errington, J .
MOLECULAR MICROBIOLOGY, 1997, 25 (05) :945-954
[28]   Identification and characterization of a bacterial chromosome partitioning site [J].
Lin, DCH ;
Grossman, AD .
CELL, 1998, 92 (05) :675-685
[29]   Bipolar localization of a chromosome partition protein in Bacillus subtilis [J].
Lin, DCH ;
Levin, PA ;
Grossman, AD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (09) :4721-4726
[30]   ANALYSIS OF STREPTOMYCES-AVERMITILIS GENES REQUIRED FOR AVERMECTIN BIOSYNTHESIS UTILIZING A NOVEL INTEGRATION VECTOR [J].
MACNEIL, DJ ;
GEWAIN, KM ;
RUBY, CL ;
DEZENY, G ;
GIBBONS, PH ;
MACNEIL, T .
GENE, 1992, 111 (01) :61-68