Mucosal and invading bacteria in patients with inflammatory bowel disease compared with controls

被引:280
作者
Kleessen, B
Kroesen, AJ
Buhr, HJ
Blaut, M
机构
[1] German Inst Human Nutr, Dept Gastrointestinal Microbiol, DE-14558 Bergholz Rehbrucke, Germany
[2] Benjamin Franklin Med Ctr, Berlin, Germany
关键词
Crohn disease; intestinal bacteria; inflammatory bowel disease; in situ hybridization; mucosa; mucin; ulcerative colitis;
D O I
10.1080/003655202320378220
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background: Endogenous intestinal bacteria and/or specific bacterial pathogens are suspected of being involved in the pathogenesis of inflammatory bowel diseases (IBD). The aim of this study was to investigate IBD tissues for different bacterial population groups harbouring the mucosal surface and/or invading the mucosa. Methods: Tissue sections from surgical resections from the terminal ileum and/or the colon from 24 IBD patients (12 active ulcerative colitis (UC), 12 active Crohn disease (CD)) and 14 non-IBD controls were studied by fluorescent in situ hybridization on a quantifiable basis. Results: More bacteria were detected on the mucosal surface of IBD patients than on those of non-IBD controls (P < 0.05). Bacterial invasion of the mucosa was evident in 83.3% of colonic specimens from the UC patients, in 55.6% of the ileal and in 25% of the colonic specimens from the CD patients, but no bacteria were detected in the tissues of the controls. Colonic UC specimens were colonized by a variety of organisms, such as bacteria belonging to the gamma subdivision of Proteobacteria, the Enterobacteriaceae, the Bacteroides/Prevotella cluster, the Clostridium histolyticum/Clostridium lituseburense group, the Clostridium coccoides/Eubacterium rectal e group, high G + C Gram-positive bacteria, or sulphate-reducing bacteria, while CD samples harboured mainly bacteria belonging to the former three groups. Conclusion: Pathogenic events in CD and UC may be associated with different alterations in the mucosal flora of the ileum and colon.
引用
收藏
页码:1034 / 1041
页数:8
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