Mangiferin Has an Additive Effect on the Apoptotic Properties of Hesperidin in Cyclopia sp. Tea Extracts

被引：51

作者：

Bartoszewski, Rafal ^{[1
]}

Hering, Anna ^{[1
]}

Marszall, Marcin ^{[4
]}

Hajduk, Justyna Stefanowicz ^{[1
]}

Bartoszewska, Sylwia ^{[2
]}

Kapoor, Niren ^{[3
]}

Kochan, Kinga ^{[1
]}

Ochocka, Renata ^{[1
]}

机构：

[1] Med Univ Gdansk, Dept Biol & Pharmaceut Bot, Gdansk, Poland

[2] Med Univ Gdansk, Dept Inorgan Chem, Gdansk, Poland

[3] Univ Alabama Birmingham, Dept Neurol, Birmingham, AL 35294 USA

[4] Med Univ Gdansk, Dept Toxicol, Gdansk, Poland

来源：

PLOS ONE | 2014年 / 9卷 / 03期

关键词：

NF-KAPPA-B; BREAST-CANCER CELLS; TIME PCR DATA; REAL-TIME; XCELLIGENCE SYSTEM; PROTEIN TRADD; RECEPTOR; ACTIVATION; PATHWAYS; GENE;

D O I：

10.1371/journal.pone.0092128

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

A variety of biological pro-health activities have been reported for mangiferin and hesperidin, two major phenolic compounds of Honeybush (Cyclopia sp.) tea extracts. Given their increasing popularity, there is a need for understanding the mechanisms underlying the biological effects of these compounds. In this study, we used real-time cytotoxicity cellular analysis of the Cyclopia sp. extracts on HeLa cells and found that the higher hesperidin content in non-fermented "green" extracts correlated with their higher cytotoxicity compared to the fermented extracts. We also found that mangiferin had a modulatory effect on the apoptotic effects of hesperidin. Quantitative PCR analysis of hesperidin-induced changes in apoptotic gene expression profile indicated that two death receptor pathway members, TRADD and TRAMP, were up regulated. The results of this study suggest that hesperidin mediates apoptosis in HeLa cells through extrinsic pathway for programmed cell death.

引用

页数：12

共 48 条

[1]

Mangiferin Attenuates Osteoclastogenesis, Bone Resorption, and RANKL-Induced Activation of NF-κB and ERK [J].