Application of atomic force microscopy to visualization of DNA, chromatin, and chromosomes

被引：28

作者：

Fritzsche, W

Takac, L

Henderson, E

机构：

[1] IOWA STATE UNIV,DEPT ZOOL & GENET,AMES,IA 50011

[2] DEBRECEN UNIV MED,SCH MED,DEPT OPHTHALMOL,H-4012 DEBRECEN,HUNGARY

来源：

CRITICAL REVIEWS IN EUKARYOTIC GENE EXPRESSION | 1997年 / 7卷 / 03期

关键词：

scanning force microscopy; atomic force microscopy; chromosomes; DNA; nucleosomes;

D O I：

10.1615/CritRevEukarGeneExpr.v7.i3.30

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The scanning force microscope (SFM, also called the atomic force microscope, AFM) provides a new and powerful method for visualization and manipulation of biological samples. Its high precision and sensitivity allow the investigator to interrogate samples at very high spatial resolution and simultaneously accumulate a variety of data types, including topography, viscoelasticity, chemical properties, and local friction. We provide here a brief review of the literature describing the current state of the art in the applicaticn of SFM to the study of DNA, chromatin and chromosomes, and some examples from this laboratory. Suggestion for future directions of this technology are also presented.

引用

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页码：231 / 240

页数：10

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