dsDNA, ssDNA, G-quadruplex DNA, and nucleosomal DNA electrochemical screening using canthin-6-one alkaloid-modified electrodes

被引：19

作者：

Domenech-Carbo, Antonio ^{[1
]}

Cebrian-Torrejon, Gerardo ^{[1
,2
]}

de Miguel, Laura ^{[3
]}

Tordera, Vicente ^{[4
]}

Rodrigues-Furtado, Daniel ^{[5
]}

Assad-Kahn, Suzana ^{[6
]}

Fournet, Alain ^{[7
]}

Figadere, Bruno ^{[2
]}

Vazquez-Manrique, Rafael P. ^{[8
,9
]}

Poupon, Erwan ^{[2
]}

机构：

[1] Univ Valencia, Dept Quim Analit, E-46100 Burjassot, Spain

[2] Univ Paris 11, CNRS, Lab Pharmacognosie, UMR 8076,BioCIS,LabEx LERMIT,Fac Pharm, F-92296 Chatenay Malabry, France

[3] Univ Paris 11, Inst Galien Paris Sud, CNRS, UMR 8612,Fac Pharm, F-92296 Chatenay Malabry, France

[4] Univ Valencia, Dept Bioquim & Biol Mol, E-46100 Burjassot, Spain

[5] Univ Fed Rio de Janeiro, Inst Biomed Sci, Natl Lab Embryon Stem Cell Res, Rio De Janeiro, Brazil

[6] Univ Fed Rio de Janeiro, Inst Ciencias Biomed, BR-21949590 Rio De Janeiro, Brazil

[7] Univ Paris 11, Inst Rech Dev IRD, UMR 217, Lab Pharmacognosie,Fac Pharm, F-92296 Chatenay Malabry, France

[8] Hlth Res Inst La Fe, Valencia, Spain

[9] Biomed Network Res Ctr Rare Dis, Valencia, Spain

来源：

ELECTROCHIMICA ACTA | 2014年 / 115卷

关键词：

Voltammetry of microparticles; DNA screening; G-quadruplex-DNA; Canthin-6-one; Natural products; DOUBLE-STRANDED DNA; ELECTROCATALYTIC ACTIVITY; CATION-BINDING; LARIAT ETHERS; REDOX; VISUALIZATION; MICROSCOPY; DAMAGE; TRIPHOSPHATES; DERIVATIVES;

D O I：

10.1016/j.electacta.2013.11.025

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

070208 [无线电物理];

摘要：

Microparticulate films of a canthin-6-one alkaloid (L), a natural beta-carboline alkaloid presenting a characteristic naphtyridone motif, on glassy carbon electrodes yield different, separate voltammetric signals for dsDNA, ssDNA, G-quadruplex DNA, different degrees of DNA methylation and the biomimetic nucleosomal DNA with detection limit of 10(-5) M. This multiple-signal electrochemical behavior is in contrast with conventional use of DNA intercalators, only discriminating between different DNA forms by variations in the intensity of a unique signal. Complementary photochemical and scanning electrochemical microscopy (SECM) data suggest that the differences in the voltammetric response of the different DNA forms derive from the intercalating properties of the canthin-6-one. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：546 / 552

页数：7

共 60 条

[1]

Bard A.J., 2001, ELECTROCHEMICAL MICR

[2]

Photoelectrochemical competitive DNA hybridization assay using semiconductor quantum dot conjugated oligonucleotides [J].

Bas, Deniz ;

Boyaci, Ismail Hakki .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2011, 400 (03) :703-707

[3]

Ferrocenylethynyl derivatives of nucleoside triphosphates:: Synthesis, incorporation, electrochemistry, and bioanalytical applications [J].

Brazdilova, Petra ;

Vrabel, Milan ;

Pohl, Radek ;

Pivonkova, Hana ;

Havran, Ludek ;

Hocek, Michal ;

Fojta, Miroslav .

CHEMISTRY-A EUROPEAN JOURNAL, 2007, 13 (34) :9527-9533

[4]

Cebrián-Torrejón G, 2013, EUR J ORG CHEM, V2013, P5821, DOI 10.1002/ejoc.201300770

[5]

Alkaloids from Rutaceae: activities of canthin-6-one alkaloids and synthetic analogues on glioblastoma stems cells [J].

Cebrian-Torrejon, Gerardo ;

Kahn, Suzana Assad ;

Elena Ferreira, Maria ;

Thirant, Cecile ;

Rojas de Arias, Antonieta ;

Figadere, Bruno ;

Fournet, Alain ;

Chneiweiss, Herve ;

Poupon, Erwan .

MEDCHEMCOMM, 2012, 3 (07) :771-774

[6]

Cosa G, 2001, PHOTOCHEM PHOTOBIOL, V73, P585, DOI 10.1562/0031-8655(2001)073<0585:PPOFDD>2.0.CO

[7]

[8]

Stimulation of topoisomerase II-mediated DNA cleavage by three DNA-intercalating plant alkaloids: Cryptolepine, matadine, and serpentine [J].

Dassonneville, L ;

Bonjean, K ;

De Pauw-Gillet, MC ;

Colson, P ;

Houssier, C ;

Quetin-Leclercq, J ;

Angenot, L ;

Bailly, C .

BIOCHEMISTRY, 1999, 38 (24) :7719-7726

[9]

G-quartets 40 years later:: From 5′-GMP to molecular biology and supramolecular chemistry [J].

Davis, JT .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (06) :668-698

[10]

Electrochemical anion sensing using electrodes chemically modified with Au(I)-Cu(I) heterotrimetallic alkynyl cluster complexes containing ferrocenyl groups [J].

Domenech, Antonio ;

Koshevoy, Igor O. ;

Montoya, Noemi ;

Pakkanen, Tapani A. .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2010, 397 (05) :2013-2022

← 1 2 3 4 5 6 →