Increased expression and altered location of annexin IV in renal clear cell carcinoma: a possible role in tumour dissemination

被引：84

作者：

Zimmermann, U

Balabanov, S

Giebel, J

Teller, S

Junker, H

Schmoll, D

Protzel, C

Scharf, C

Kleist, B

Walther, R

机构：

[1] Univ Klinikum Greifswald, Dept Med Biochem & Mol Biol, D-17487 Greifswald, Germany

[2] Ernst Moritz Arndt Univ Greifswald, Dept Urol, D-17487 Greifswald, Germany

[3] Ernst Moritz Arndt Univ Greifswald, Dept Anat, D-17487 Greifswald, Germany

[4] Dept Microbiol & Mol Biol, D-17487 Greifswald, Germany

[5] Ernst Moritz Arndt Univ Greifswald, Dept Pathol, D-17487 Greifswald, Germany

来源：

CANCER LETTERS | 2004年 / 209卷 / 01期

关键词：

kidney cancer; kidney tissue; annexin IV; proteome; RT-PCR; immunohistochemistry; cell migration;

D O I：

10.1016/j.canlet.2003.12.002

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The proteome of renal cell carcinoma and non-neoplastic kidney tissue was analysed from 12 patients by two-dimensional polyacrylamide gel electrophoresis to search for differentially expressed proteins in the tumour. Annexin IV was identified to be up-regulated in tumour cells. These patients and further II were characterized by RT-PCR. We found an increased amount of annexin IV mRNA. Immunohistochemical analysis revealed an altered localization of annexin IV in tumour cells. Additionally we demonstrate that over-expressed annexin IV promotes cell migration in a carcinoma model system. From these results above it seems possible that annexin IV plays an important role in the morphological diversification and dissemination of the clear cell renal cell carcinoma. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

引用

页码：111 / 118

页数：8

共 24 条

[1] Identification of proteins in human prostate tumor material by two-dimensional gel electrophoresis and mass spectrometry [J].

Alaiya, AA ;

Oppermann, M ;

Langridge, J ;

Roblick, U ;

Egevad, L ;

Brändstedt, S ;

Hellström, M ;

Linder, S ;

Bergman, T ;

Jörnvall, H ;

Auer, G .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2001, 58 (02) :307-311

[2] Tumour-related enzyme alterations in the clear cell type of human renal cell carcinoma identified by two-dimensional gel electrophoresis [J].

Balabanov, S ;

Zimmermann, U ;

Protzel, C ;

Scharf, C ;

Klebingat, KJ ;

Walther, R .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2001, 268 (22) :5977-5980

[3] Insulin-like growth factor receptor cooperates with integrin alpha v beta 5 to promote tumor cell dissemination in vivo [J].

Brooks, PC ;

Klemke, RL ;

Schon, S ;

Lewis, JM ;

Schwartz, MA ;

Cheresh, DA .

JOURNAL OF CLINICAL INVESTIGATION, 1997, 99 (06) :1390-1398

[4] Activated leukocyte cell adhesion molecule (ALCAM) and annexin II are involved in the metastatic progression of tumor cells after chemotherapy with Adriamycin [J].

Choi, S ;

Kobayashi, M ;

Wang, JX ;

Habelhah, H ;

Okada, F ;

Hamada, J ;

Moriuchi, T ;

Totsuka, Y ;

Hosokawa, M .

CLINICAL & EXPERIMENTAL METASTASIS, 2000, 18 (01) :45-50

[5] Differential expression of annexins I, II and IV in human tissues: an immunohistochemical study [J].

Dreier, R ;

Schmid, KW ;

Gerke, V ;

Riehemann, K .

HISTOCHEMISTRY AND CELL BIOLOGY, 1998, 110 (02) :137-148

[6] Changes in the expression of alpha(v) integrins in oral squamous cell carcinomas [J].

Jones, J ;

Watt, FM ;

Speight, PM .

JOURNAL OF ORAL PATHOLOGY & MEDICINE, 1997, 26 (02) :63-68

[7]

KAETZEL MA, 1994, J BIOL CHEM, V269, P5297

[8]

Kang JS, 2002, CLIN CANCER RES, V8, P117

[9] Biophysical and molecular properties of annexin-formed channels [J].

Kourie, JI ;

Wood, HB .

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 2000, 73 (2-4) :91-134

[10] IDENTIFICATION OF THE VONHIPPEL-LINDAU DISEASE TUMOR-SUPPRESSOR GENE [J].

LATIF, F ;

TORY, K ;

GNARRA, J ;

YAO, M ;

DUH, FM ;

ORCUTT, ML ;

STACKHOUSE, T ;

KUZMIN, I ;

MODI, W ;

GEIL, L ;

SCHMIDT, L ;

ZHOU, FW ;

LI, H ;

WEI, MH ;

CHEN, F ;

GLENN, G ;

CHOYKE, P ;

WALTHER, MM ;

WENG, YK ;

DUAN, DSR ;

DEAN, M ;

GLAVAC, D ;

RICHARDS, FM ;

CROSSEY, PA ;

FERGUSONSMITH, MA ;

LEPASLIER, D ;

CHUMAKOV, I ;

COHEN, D ;

CHINAULT, AC ;

MAHER, ER ;

LINEHAN, WM ;

ZBAR, B ;

LERMAN, MI .

SCIENCE, 1993, 260 (5112) :1317-1320

← 1 2 3 →