Langerhans cells exhibit low responsiveness to double-stranded RNA

被引：25

作者：

Fujita, H ^{[1
]}

Asahina, A ^{[1
]}

Mitsui, H ^{[1
]}

Tamaki, K ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Med, Dept Dermatol, Tokyo, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 319卷 / 03期

关键词：

toll-like receptor 3; poly(I : C); chemokine; cytokine; keratinocyte;

D O I：

10.1016/j.bbrc.2004.05.059

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Double-stranded RNA (dsRNA) is a viral product recognized by Toll-like receptor 3 (TLR3), and it is a potent activator of dendritic cells (DC). We compared Langerhans cells (LC) and splenic CD11c(+) DC and investigated the responsiveness to dsRNA. We prepared highly purified LC (>95%) using the panning method. TLR3 mRNA was expressed in LC, splenic DC, and keratinocytes (KC). The expression of IFN-beta mRNA was enhanced in LC and splenic DC by Poly(I:C) stimulation. However, cytokine/chemokine production in response to Poly(I:C) by LC was much lower than that by splenic DC. In addition, Poly(I:C) induced further maturation in splenic DC, but not in LC. Finally, we found that the mouse KC cell line, PAM212, produced a great amount of IL-1alpha by Poly(I:C) stimulation, and that IL-1alpha promoted the maturation of LC. These data altogether indicate that LC exhibit low responsiveness to dsRNA. It is possible that KC may primarily trigger anti-viral immune responses in the skin via cytokine production such as IL-1alpha. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：832 / 839

页数：8

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