Identification of dimethylarsinoyl-riboside derivatives in seaweed by pneumatically assisted electrospray tandem mass spectrometry

被引：56

作者：

McSheehy, S ^{[1
]}

Marcinek, M ^{[1
]}

Chassaigne, H ^{[1
]}

Szpunar, J ^{[1
]}

机构：

[1] CNRS, EP132, F-64053 Pau, France

来源：

ANALYTICA CHIMICA ACTA | 2000年 / 410卷 / 1-2期

关键词：

speciation; arsenosugars; algae; HPLC-ICP MS; ES MS/MS;

D O I：

10.1016/S0003-2670(00)00706-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Pneumatically-assisted electrospray tandem mass spectrometry (ES MS/MS) was proposed as a technique for the identification of dimethylarsinoyl-riboside derivatives (arsenosugars) in seaweed (Laminaria). Conditions for the acquisition of MS and MS/MS spectra were optimized. A size-exclusion HPLC (SE HPLC) step was developed for the purification of algal extracts prior to ES MS. The arsenosugar fraction was found to elute prior to the majority of other arsenic compounds. The identity of the compounds expected to be arsenosugar compounds was confirmed by the collision induced dissociation (CID) of the relevant protonated molecule ions. An independent confirmation of the identity of analytes was obtained by two-dimensional (size-exclusion-anion exchange) HPLC-ICP MS with signal identification by spiking with the appropriate arsenosugar standards. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：71 / 84

页数：14

共 33 条

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