Pseudophosphorylated prolactin (S179D PRL) inhibits growth and promotes β-casein gene expression in the rat mammary gland

被引：32

作者：

Kuo, CB ^{[1
]}

Wu, W ^{[1
]}

Xu, XL ^{[1
]}

Yang, LL ^{[1
]}

Chen, C ^{[1
]}

Coss, D ^{[1
]}

Birdsall, B ^{[1
]}

Nasseri, D ^{[1
]}

Walker, AM ^{[1
]}

机构：

[1] Univ Calif Riverside, Div Biomed Sci, Riverside, CA 92521 USA

来源：

CELL AND TISSUE RESEARCH | 2002年 / 309卷 / 03期

关键词：

unmodified prolactin; phosphorylated prolactin; duct; alveolus; beta casein; rat (Sprague Dawley);

D O I：

10.1007/s00441-002-0598-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have investigated the individual roles of unmodified prolactin (U-PRL) and a mimic of phosphorylated PRL (S179D PRL) in mammary development. Recombinant versions of the PRLs were delivered to rats throughout pregnancy at a rate of 6 mug/24 h per rat and to non-pregnant females at a rate of 24 mug/24 h per rat. Measurement of progesterone, corticosterone, and estradiol showed no effect of the administered PRLs on the levels of these other mammotropic hormones. Histological and morphometric analysis showed U-PRL to cause mammary growth, whereas S179D PRL inhibited growth. Molecular analysis demonstrated decreased beta-casein expression in the mammary glands of the U-PRL-treated animals at term and increased beta-casein expression in the mammary glands of the S179D PRL-treated animals. Superior beta-casein gene expression in response to S179D PRL versus U-PRL was confirmed in HC11 cells. We conclude that U-PRL is important for growth, whereas S179D PRL promotes at least one measure of differentiated function in the mammary gland.

引用

页码：429 / 437

页数：9

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