Hydroquinone:O-glucosyltransferase from cultivated Rauvolfia cells:: enrichment and partial amino acid sequences

被引：18

作者：

Arend, J ^{[1
]}

Warzecha, H ^{[1
]}

Stöckigt, J ^{[1
]}

机构：

[1] Johannes Gutenberg Univ Mainz, Inst Pharm, Dept Pharmaceut Biol, D-55099 Mainz, Germany

来源：

PHYTOCHEMISTRY | 2000年 / 53卷 / 02期

关键词：

Rauvolfia serpentina cell culture; apocynaceae; hydroquinone; uridine-diphosphate-glucose transferase; arbutin; partial purification; partial amino acid sequence;

D O I：

10.1016/S0031-9422(99)00539-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plant cell suspension cultures of Rauvolfia are able to produce a high amount of arbutin by glucosylation of exogenously added hydroquinone. A four step purification procedure using anion exchange, hydrophobic interaction, hydroxyapatite-chromatography and chromatofocusing delivered in a yield of 0.5%, an approximately 390 fold enrichment of the involved glucosyltransferase. SDS-PAGE showed a M-r for the enzyme of 52 kDa. Proteolysis of the pure enzyme with endoproteinase LysC revealed six peptide fragments with 9-23 amino acids which were sequenced. Sequence alignment of the six peptides showed high homolog ies to glycosyltransferases from other higher plants. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：187 / 193

页数：7

共 24 条

[1]

Akiu S., 1988, P JPN SOC DERMATOL, V12, P138

[2]

Bandurski Robert S., 1995, P39

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] MOLECULAR-CLONING, EXPRESSION, AND INDUCTION OF BERBERINE BRIDGE ENZYME, AN ENZYME ESSENTIAL TO THE FORMATION OF BENZOPHENANTHRIDINE ALKALOIDS IN THE RESPONSE OF PLANTS TO PATHOGENIC ATTACK [J].

DITTRICH, H ;

KUTCHAN, TM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (22) :9969-9973

[5] Cloning and characterization of Vitis vinifera UDP-glucose:flavonoid 3-O-glucosyltransferase, a homologue of the enzyme encoded by the maize Bronze-1 locus that may primarily serve to glucosylate anthocyanidins in vivo [J].

Ford, CM ;

Boss, PK ;

Hoj, PB .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (15) :9224-9233

[6] PARTIAL-PURIFICATION AND PROPERTIES OF UDP-GLUCOSE - VANILLATE 1-O-GLUCOSYL TRANSFERASE FROM OAK LEAVES [J].

GROSS, GG .

PHYTOCHEMISTRY, 1983, 22 (10) :2179-2182

[7] SIMPLIFIED METHOD FOR SILVER STAINING OF PROTEINS IN POLYACRYLAMIDE GELS AND THE MECHANISM OF SILVER STAINING [J].

HEUKESHOVEN, J ;

DERNICK, R .

ELECTROPHORESIS, 1985, 6 (03) :103-112

[8] MULTIPLE SECONDARY PLANT PRODUCT UDP-GLUCOSE GLUCOSYLTRANSFERASE GENES EXPRESSED IN CASSAVA (MANIHOT-ESCULENTA CRANTZ) COTYLEDONS [J].

HUGHES, J ;

HUGHES, MA .

DNA SEQUENCE, 1994, 5 (01) :41-49

[9]

INOMATA S, 1991, APPL MICROBIOL BIOT, V36, P315, DOI 10.1007/BF00208148

[10]

Kutchan T. M., 1998, The Alkaloids, V50, P257

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