Hydroquinone:O-glucosyltransferase from cultivated Rauvolfia cells:: enrichment and partial amino acid sequences

被引：18

作者：

Arend, J ^{[1
]}

Warzecha, H ^{[1
]}

Stöckigt, J ^{[1
]}

机构：

[1] Johannes Gutenberg Univ Mainz, Inst Pharm, Dept Pharmaceut Biol, D-55099 Mainz, Germany

来源：

PHYTOCHEMISTRY | 2000年 / 53卷 / 02期

关键词：

Rauvolfia serpentina cell culture; apocynaceae; hydroquinone; uridine-diphosphate-glucose transferase; arbutin; partial purification; partial amino acid sequence;

D O I：

10.1016/S0031-9422(99)00539-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plant cell suspension cultures of Rauvolfia are able to produce a high amount of arbutin by glucosylation of exogenously added hydroquinone. A four step purification procedure using anion exchange, hydrophobic interaction, hydroxyapatite-chromatography and chromatofocusing delivered in a yield of 0.5%, an approximately 390 fold enrichment of the involved glucosyltransferase. SDS-PAGE showed a M-r for the enzyme of 52 kDa. Proteolysis of the pure enzyme with endoproteinase LysC revealed six peptide fragments with 9-23 amino acids which were sequenced. Sequence alignment of the six peptides showed high homolog ies to glycosyltransferases from other higher plants. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：187 / 193

页数：7

共 24 条

[11] EXPRESSION OF ENZYMATICALLY ACTIVE CLONED STRICTOSIDINE SYNTHASE FROM THE HIGHER-PLANT RAUVOLFIA-SERPENTINA IN ESCHERICHIA-COLI [J].

KUTCHAN, TM .

FEBS LETTERS, 1989, 257 (01) :127-130

[12] ORGANIC GROWTH FACTOR REQUIREMENTS OF TOBACCO TISSUE CULTURES [J].

LINSMAIER, EM ;

SKOOG, F .

PHYSIOLOGIA PLANTARUM, 1965, 18 (01) :100-+

[13] HIGH-YIELD FORMATION OF ARBUTIN FROM HYDROQUINONE BY CELL-SUSPENSION CULTURES OF RAUWOLFIA-SERPENTINA [J].

LUTTERBACH, R ;

STOCKIGT, J .

HELVETICA CHIMICA ACTA, 1992, 75 (06) :2009-2011

[14] EFFECT OF SUBSTITUENT GROUPS ON THE GLUCOSYL CONJUGATION OF XENOBIOTIC PHENOLS BY CULTURED-CELLS OF GARDENIA-JASMINOIDES [J].

MIZUKAMI, H ;

HIRANO, A ;

OHASHI, H .

PLANT SCIENCE, 1987, 48 (01) :11-15

[15] CHARACTERIZATION OF O-GLUCOSYLTRANSFERASES WITH ACTIVITIES TOWARD PHENOLIC SUBSTRATES IN ALFALFA [J].

PARRY, AD ;

EDWARDS, R .

PHYTOCHEMISTRY, 1994, 37 (03) :655-661

[16] IMPROVED TOOLS FOR BIOLOGICAL SEQUENCE COMPARISON [J].

PEARSON, WR ;

LIPMAN, DJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (08) :2444-2448

[17] Free and glucosylated phenolics, phenol beta-glucosyltransferase activity and membrane permeability in cucumber roots affected by derivatives of cinnamic and benzoic acids [J].

Politycka, B .

ACTA PHYSIOLOGIAE PLANTARUM, 1997, 19 (03) :311-317

[18]

STEGLICH W, 1997, ROMPP LEXIKON NATURS, P53

[19] POTENTIAL OF DATURA-INNOXIA CELL-SUSPENSION CULTURES FOR GLUCOSYLATING HYDROQUINONE [J].

SUZUKI, T ;

YOSHIOKA, T ;

TABATA, M ;

FUJITA, Y .

PLANT CELL REPORTS, 1987, 6 (04) :275-278

[20] GLUCOSYLATION OF PHENOLIC-COMPOUNDS BY PLANT-CELL CULTURES [J].

TABATA, M ;

UMETANI, Y ;

OOYA, M ;

TANAKA, S .

PHYTOCHEMISTRY, 1988, 27 (03) :809-813

← 1 2 3 →