Systemic, nasal and oral live vaccines against Pseudomonas aeruginosa: A clinical trial of immunogenicity in lower airways of human volunteers

被引：42

作者：

Bumann, Dirk ^{[3
]}

Behre, Christoph ^{[2
]}

Behre, Katharina ^{[2
]}

Herz, Steffen ^{[2
]}

Gewecke, Britta ^{[1
]}

Gessner, J. Engelbert

von Specht, Bernd Ulrich ^{[2
]}

Baumann, Ulrich ^{[1
]}

机构：

[1] Hannover Med Sch, Dept Paediat Pulmonol & Neonatol, D-30623 Hannover, Germany

[2] Univ Freiburg, Clin Res Ctr, Freiburg, Germany

[3] Hannover Med Sch, Dept Immunol, Jr Res Grp Mucosal Immunol, D-30623 Hannover, Germany

来源：

VACCINE | 2010年 / 28卷 / 03期

关键词：

Mucosal vaccines; Nasal vaccination; Oral vaccination; Pseudomonas vaccine; OprF-OprI; CVD908; Ty21a; OUTER-MEMBRANE PROTEIN; ENTERICA SEROVAR TYPHI; F-I VACCINE; OPRF-OPRI; INTRANASAL IMMUNIZATION; MUCOSAL VACCINATION; HELICOBACTER-PYLORI; ANTIBODY-RESPONSES; RESPIRATORY-TRACT; CYSTIC-FIBROSIS;

D O I：

10.1016/j.vaccine.2009.10.080

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

071005 [微生物学]; 100108 [医学免疫学];

摘要：

Vaccination against Pseudomonas aeruginosa is a desirable, yet challenging strategy for prevention of airway infection in patients with cystic fibrosis. We compared the formation of antibodies in lower airways induced by systemic and mucosal vaccination strategies. We immunised 48 volunteers in six vaccination groups with either a systemic, a nasal, or four newly constructed oral live vaccines based on attenuated live Salmonella (strains CVD908 and Ty21a). followed by a systemic booster vaccination All vaccines were based on a recombinant fusion protein of the highly conserved P aeruginosa outer membrane proteins OprF and OprI as antigen. While systemic and mucosal vaccines induced a comparable rise of serum antibody titers, a significant rise of IgA and IgG antibodies in the lower airways was noted only after nasal and oral vaccinations. We conclude that nasal and oral OprF-OprI vaccines are promising candidates for development of antipseudomonal immunisation through inducing a specific antibody response in the lung. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：707 / 713

页数：7

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