Structural constraints from proton-mediated rare-spin correlation spectroscopy in rotating solids

被引:220
作者
Lange, A [1 ]
Luca, S [1 ]
Baldus, M [1 ]
机构
[1] Max Planck Inst Biophys Chem, Solid State NMR, Dept NMR Based Struct Biol, D-37077 Gottingen, Germany
关键词
D O I
10.1021/ja026691b
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A novel solid-state NMR concept is introduced that allows for the detection of through-space proton-proton contacts in high spectral resolution. The proposed method not only can be utilized to infer sequential assignments and backbone or side chain conformation in solid-phase polypeptides, but it also provides information about the three-dimensional arrangement of the molecule of interest. As a result, the molecular structure can be studied without additional restrictions regarding sample labeling or magic angle spinning rates. Copyright © 2002 American Chemical Society.
引用
收藏
页码:9704 / 9705
页数:2
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