Sequence completion, identification and definition of the fengycin operon in Bacillus subtilis 168

被引：71

作者：

Tosato, V

Albertini, AM

Zotti, M

Sonda, S

Bruschi, CV

机构：

[1] ITALTBS RIC SRL,AREA SCI PK,I-34012 TRIESTE,ITALY

[2] INT CTR GENET ENGN & BIOTECHNOL,MICROBIOL GRP,I-34012 TRIESTE,ITALY

[3] UNIV PAVIA,DIPARTIMENTO GENET & MICROBIOL A BUZZATI TRAVERS,I-27100 PAVIA,ITALY

来源：

MICROBIOLOGY-UK | 1997年 / 143卷

关键词：

antifungal antibiotic; Bacillus subtilis; fengycin; lipopeptide synthetase;

D O I：

10.1099/00221287-143-11-3443

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A 15 kb DNA fragment from the Bacillus subtilis chromosome between citB and ppsC has been sequenced, and new ORFs encoding putative enzymes involved in lipopolypeptide synthesis, which complete a partial operon previously reported, and a new set of enzymes responsible for lipid metabolism have been identified. From the analysis of DNA sequence homology of the fragment it was deduced that these new peptide synthetase genes are part of an operon for the biosynthesis of the fungicide fengycin.

引用

收藏

页码：3443 / 3450

页数：8

相关论文

共 34 条

[1]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[2] INTRINSIC AND EXTRINSIC APPROACHES FOR DETECTING GENES IN A BACTERIAL GENOME [J].

BORODOVSKY, M ;

RUDD, KE ;

KOONIN, EV .

NUCLEIC ACIDS RESEARCH, 1994, 22 (22) :4756-4767

[3] TRANSPOSON MUTAGENESIS AND CLONING OF THE GENES ENCODING THE ENZYMES OF FENGYCIN BIOSYNTHESIS IN BACILLUS-SUBTILIS [J].

CHEN, CL ;

CHANG, LK ;

CHANG, YS ;

LIU, ST ;

TSCHEN, JSM .

MOLECULAR & GENERAL GENETICS, 1995, 248 (02) :121-125

[4] SEQUENCE AND ANALYSIS OF THE GENETIC-LOCUS RESPONSIBLE FOR SURFACTIN SYNTHESIS IN BACILLUS-SUBTILIS [J].

COSMINA, P ;

RODRIGUEZ, F ;

DEFERRA, F ;

GRANDI, G ;

PEREGO, M ;

VENEMA, G ;

VANSINDEREN, D .

MOLECULAR MICROBIOLOGY, 1993, 8 (05) :821-831

[5] A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].

DEVEREUX, J ;

HAEBERLI, P ;

SMITHIES, O .

NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395

[6] BACILLUS-SUBTILIS GENOME PROJECT - CLONING AND SEQUENCING OF THE 97KB REGION FROM 325-DEGREES TO 333 DEGREES [J].

GLASER, P ;

KUNST, F ;

ARNAUD, M ;

COUDART, MP ;

GONZALES, W ;

HULLO, MF ;

IONESCU, M ;

LUBOCHINSKY, B ;

MARCELINO, L ;

MOSZER, I ;

PRESECAN, E ;

SANTANA, M ;

SCHNEIDER, E ;

SCHWEIZER, J ;

VERTES, A ;

RAPOPORT, G ;

DANCHIN, A .

MOLECULAR MICROBIOLOGY, 1993, 10 (02) :371-384

[7]

Hanahan D., 1985, DNA CLONING, VI, P109

[8] COMPLETE PHYSICAL MAP OF THE BACILLUS-SUBTILIS 168-CHROMOSOME CONSTRUCTED BY A GENE-DIRECTED MUTAGENESIS METHOD [J].

ITAYA, M ;

TANAKA, T .

JOURNAL OF MOLECULAR BIOLOGY, 1991, 220 (03) :631-648

[9] NONRIBOSOMAL BIOSYNTHESIS OF PEPTIDE ANTIBIOTICS [J].

KLEINKAUF, H ;

VONDOHREN, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 192 (01) :1-15

[10] POSITIVE REGULATION IN THE GRAM-POSITIVE BACTERIUM - BACILLUS-SUBTILIS [J].

KLIER, A ;

MSADEK, T ;

RAPOPORT, G .

ANNUAL REVIEW OF MICROBIOLOGY, 1992, 46 :429-459

← 1 2 3 4 →