Analysing immune cell migration

被引:159
作者
Beltman, Joost B. [1 ]
Maree, Athanasius F. M. [2 ]
de Boer, Rob J. [1 ]
机构
[1] Univ Utrecht, NL-3584 CH Utrecht, Netherlands
[2] John Innes Ctr, Norwich NR4 7UH, Norfolk, England
基金
英国生物技术与生命科学研究理事会;
关键词
LYMPH-NODE CORTEX; DENDRITIC CELLS; T-CELLS; 2-PHOTON MICROSCOPY; IN-VIVO; NETWORKS; DYNAMICS; MOTILITY; ANTIGEN; SYSTEM;
D O I
10.1038/nri2638
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The visualization of the dynamic behaviour of and interactions between immune cells using time-lapse video microscopy has an important role in modern immunology. To draw robust conclusions, quantification of such cell migration is required. However, imaging experiments are associated with various artefacts that can affect the estimated positions of the immune cells under analysis, which form the basis of any subsequent analysis. Here, we describe potential artefacts that could affect the interpretation of data sets on immune cell migration. We propose how these errors can be recognized and corrected, and suggest ways to prevent the data analysis itself leading to biased results.
引用
收藏
页码:789 / 798
页数:10
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