Three-dimensional analysis of subchondral cysts in hip osteoarthritis: An ex vivo HR-pQCT study

Introduction: Subchondral cysts are deeply related to the pathogenesis of osteoarthritis (OA), but the factors contributing to cyst formation are not well known. A three-dimensional analysis of subchondral cysts at the micro-structural level was conducted using a high-resolution peripheral quantitative Cr (HR-pQCT), and their relationships with cartilage attrition and subchondral bone microstructure were investigated. Methods: Femoral heads extracted from ten female patients with hip OA were scanned using an HR-pQCT at a voxel size of 41 mu m. The volume fractions, numbers, and sizes of the cysts were measured in the subchondral bone region under the area of cartilage loss. Furthermore, the areas of cartilage loss, as well as the microstructure of the subchondral bones, were also measured, and their correlations with the cysts were analyzed. Results: The volume fractions of cysts within subchondral bone regions varied from 2% to 33%, the numbers of cysts varied from 6 to 87, and the sizes varied from 1 mm(3) to 657 mm(3). There was a positive correlation between the number of cysts and bone volume (r > 0.8, p < 0.01). Conclusion: The degree of cyst formation showed a wide distribution in number and volume, and there was a close relationship between multiple cyst formation and bone sclerosis, which might be caused by reactive bone formation that occurred around each cyst. (C) 2014 Elsevier Inc. All rights reserved.