Nematode molecular diagnostics: From bands to barcodes

被引：139

作者：

Powers, T ^{[1
]}

机构：

[1] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA

来源：

ANNUAL REVIEW OF PHYTOPATHOLOGY | 2004年 / 42卷

关键词：

DNA barcode; plant parasitic nematodes; identification; 18S ribosomal DNA; internally transcribed spacer;

D O I：

10.1146/annurev.phyto.42.040803.140348

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Nematodes are considered among the most difficult animals to identify. DNA-based diagnostic methods have already gained acceptance in applications ranging from quarantine determinations to assessments of biodiversity. Researchers are currently in an information-gathering mode, with intensive efforts applied to accumulating nucleotide sequence of 18S and 28S ribosomal genes, internally transcribed spacer regions, and mitochondrial genes. Important linkages with collateral data such as digitized images, video clips and specimen voucher web pages are being established on GenBank and NemATOL, the nematode-specific Tree of Life database. The growing DNA taxonomy of nematodes has lead to their use in testing specific short sequences of DNA as a "barcode" for the identification of all nematode species.

引用

页码：367 / 383

页数：17