The mir-84 and let-7 paralogous microRNA genes of Caenorhabditis elegans direct the cessation of molting via the conserved nuclear hormone receptors NHR-23 and NHR-25

被引:82
作者
Hayes, Gabriel D.
Frand, Alison R.
Ruvkun, Gary [1 ]
机构
[1] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02114 USA
[2] Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
来源
DEVELOPMENT | 2006年 / 133卷 / 23期
关键词
miRNAs; microRNAs; mir-84; let-7; heterochronic pathway; molting; nuclear hormone receptors; NHR-23; NHR-25;
D O I
10.1242/dev.02655
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The let-7 microRNA ( miRNA) gene of Caenorhabditis elegans controls the timing of developmental events. let-7 is conserved throughout bilaterian phylogeny and has multiple paralogs. Here, we show that the paralog mir-84 acts synergistically with let-7 to promote terminal differentiation of the hypodermis and the cessation of molting in C. elegans. Loss of mir-84 exacerbates phenotypes caused by mutations in let-7, whereas increased expression of mir-84 suppresses a let-7 null allele. Adults with reduced levels of mir-84 and let-7 express genes characteristic of larval molting as they initiate a supernumerary molt. mir-84 and let-7 promote exit from the molting cycle by regulating targets in the heterochronic pathway and also nhr-23 and nhr-25, genes encoding conserved nuclear hormone receptors essential for larval molting. The synergistic action of miRNA paralogs in development may be a general feature of the diversified miRNA gene family.
引用
收藏
页码:4631 / 4641
页数:11
相关论文
共 67 条
[1]   The let-7 microRNA family members mir-48, mir-84, and mir-241 function together to regulate developmental timing in Caenorhabditis elegans [J].
Abbott, AL ;
Alvarez-Saavedra, E ;
Miska, EA ;
Lau, NC ;
Bartel, DP ;
Horvitz, HR ;
Ambros, V .
DEVELOPMENTAL CELL, 2005, 9 (03) :403-414
[2]   The Caenorhabditis elegans hunchback-like gene lin-57/hbl-1 controls developmental time and is regulated by microRNAs [J].
Abrahante, JE ;
Daul, AL ;
Li, M ;
Volk, ML ;
Tennessen, JM ;
Miller, EA ;
Rougvie, AE .
DEVELOPMENTAL CELL, 2003, 4 (05) :625-637
[3]  
Abrahante JE, 1998, GENETICS, V149, P1335
[4]   Evidence for a clade of nematodes, arthropods and other moulting animals [J].
Aguinaldo, AMA ;
Turbeville, JM ;
Linford, LS ;
Rivera, MC ;
Garey, JR ;
Raff, RA ;
Lake, JA .
NATURE, 1997, 387 (6632) :489-493
[5]   A HIERARCHY OF REGULATORY GENES CONTROLS A LARVA-TO-ADULT DEVELOPMENTAL SWITCH IN C-ELEGANS [J].
AMBROS, V .
CELL, 1989, 57 (01) :49-57
[6]   HETEROCHRONIC MUTANTS OF THE NEMATODE CAENORHABDITIS-ELEGANS [J].
AMBROS, V ;
HORVITZ, HR .
SCIENCE, 1984, 226 (4673) :409-416
[7]   MicroRNAs and other tiny endogenous RNAs in C-elegans [J].
Ambros, V ;
Lee, RC ;
Lavanway, A ;
Williams, PT ;
Jewell, D .
CURRENT BIOLOGY, 2003, 13 (10) :807-818
[8]   The conserved nuclear receptor Ftz-F1 is required for embryogenesis, moulting and reproduction in Caenorhabditis elegans [J].
Asahina, M ;
Ishihara, T ;
Jindra, M ;
Kohara, Y ;
Katsura, I ;
Hirose, S .
GENES TO CELLS, 2000, 5 (09) :711-723
[9]   Regulation by let-7 and lin-4 miRNAs results in target mRNA degradation [J].
Bagga, S ;
Bracht, J ;
Hunter, S ;
Massirer, K ;
Holtz, J ;
Eachus, R ;
Pasquinelli, AE .
CELL, 2005, 122 (04) :553-563
[10]   MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].
Bartel, David P. .
CELL, 2007, 131 (04) :11-29