Rapid extraction of DNA and rRNA from sediments by a novel hydroxyapatite spin-column method

被引：131

作者：

Purdy, KJ

Embley, TM

Takii, S

Nedwell, DB

机构：

[1] NAT HIST MUSEUM,DEPT ZOOL,LONDON SW7 5BD,ENGLAND

[2] TOKYO METROPOLITAN UNIV,DEPT BIOL,HACHIOJI,TOKYO 19203,JAPAN

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1996年 / 62卷 / 10期

关键词：

D O I：

10.1128/AEM.62.10.3905-3907.1996

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe a novel hydroxyapatite spin-column method of nucleic acid extraction from natural sediments by which DNA and rRNA can be extracted separately. This very rapid method produces pure nucleic acid that can be utilized in some of the most common molecular biological procedures used in the analysis of natural microbial communities.

引用

页码：3905 / 3907

页数：3

共 20 条

[1] CHROMATOGRAPHY OF NUCLEIC ACIDS ON HYDROXYAPATITE
BERNARDI, G
[J]. NATURE, 1965, 206 (4986) : 779 - &
[2] Bernardi G., 1971, METH ENZYMOL, V21, P95
[3] AMPLIFICATION OF DNA FROM NATIVE POPULATIONS OF SOIL BACTERIA BY USING THE POLYMERASE CHAIN-REACTION
BRUCE, KD
HIORNS, WD
HOBMAN, JL
OSBORN, AM
STRIKE, P
RITCHIE, DA
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (10) : 3413 - 3416
[4] DNA PROBE METHOD FOR THE DETECTION OF SPECIFIC MICROORGANISMS IN THE SOIL BACTERIAL COMMUNITY
HOLBEN, WE
JANSSON, JK
CHELM, BK
TIEDJE, JM
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (03) : 703 - 711
[5] COMPARISON OF METHODS OF DNA EXTRACTION FROM STREAM SEDIMENTS
LEFF, LG
DANA, JR
MCARTHUR, JV
SHIMKETS, LJ
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (03) : 1141 - 1143
[6] BASIS OF FRACTIONATION OF SINGLE-STRANDED NUCLEIC-ACIDS ON HYDROXYLAPATITE
MARTINSON, HG
[J]. BIOCHEMISTRY, 1973, 12 (14) : 2731 - 2736
[7] ROLE OF DOUBLE-STRANDED NUCLEIC-ACID BACKBONE CONFIGURATION IN ADSORPTION INTERACTIONS
MARTINSON, HG
[J]. BIOCHEMISTRY, 1973, 12 (14) : 2737 - 2746
[8] DIRECT EXTRACTION AND PURIFICATION OF RIBOSOMAL-RNA FOR ECOLOGICAL-STUDIES
MORAN, MA
TORSVIK, VL
TORSVIK, T
HODSON, RE
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (03) : 915 - 918
[9] MORE MI, 1994, APPL ENVIRON MICROB, V60, P1572
[10] NEDWELL DB, 1978, ESTUAR COAST MAR SCI, V6, P341, DOI 10.1016/0302-3524(78)90126-3

← 1 2 →