Sinomenine reverses multidrug resistance in bladder cancer cells via P-glycoprotein-dependent and independent manners

被引：21

作者：

Chen, Yule ^{[1
]}

Zhang, Linlin ^{[1
,3
]}

Lu, Xinlan ^{[2
]}

Wu, Kaijie ^{[1
]}

Zeng, Jin ^{[1
]}

Gao, Yang ^{[1
]}

Shi, Qi ^{[1
]}

Wang, Xinyang ^{[3
]}

Chang, Luke S. ^{[1
]}

He, Dalin ^{[1
,3
]}

机构：

[1] Xi An Jiao Tong Univ, Affiliated Hosp 1, Dept Urol, Xian 710061, Peoples R China

[2] Xi An Jiao Tong Univ, Affiliated Hosp 1, Dept Gastroenterol, Xian 710061, Peoples R China

[3] Minist Educ, Key Lab Environm & Genes Related Dis, Oncol Res Lab, Xian, Peoples R China

来源：

PHARMAZIE | 2014年 / 69卷 / 01期

基金：

中国国家自然科学基金;

关键词：

GENE-EXPRESSION; DRUG-RESISTANCE; MDR1; GENE; IN-VITRO; APOPTOSIS; ACTIVATION; CARCINOMA; CHEMORESISTANCE; PROLIFERATION; CHEMOTHERAPY;

D O I：

10.1619/ph.2014.3722

中图分类号：

R914 [药物化学];

学科分类号：

100705 [微生物与生化药学];

摘要：

P-Glycoprotein-mediated multidrug resistance is a frequent event during chemotherapy and a key obstacle for bladder cancer therapy. Search for strategies to reverse multidrug resistance is a promising approach to improve the management of bladder cancer. In the present study, we reported a novel P-glycoprotein-mediated multidrug resistant cell model 253J/DOX, which was generated from human bladder cancer 253J cell line. Furthermore, we found that the multidrug resistant phenotype of 253J/DOX cells could be overcome by sinomenine, an alkaloid derived from the stem of Sinomenium acutum. Mechanistically, the chemosensitive effect by sinomenine was mediated by down-regulating P-glycoprotein expression, as well as triggering apoptotic pathways. The chemosensitive effect of sinomenine may make it a prime candidate agent to target bladder cancer.

引用

收藏

页码：48 / 54

页数：7

相关论文

共 21 条

[1]

Increase in morphine antinociceptive activity by a P-glycoprotein inhibitor in cisplatin-induced neuropathy [J].

Balayssac, David ;

Cayre, Anne ;

Ling, Bing ;

Maublant, Jean ;

Penault-Llorca, Frederique ;

Eschalier, Alain ;

Coudore, Francois ;

Authier, Nicolas .

NEUROSCIENCE LETTERS, 2009, 465 (01) :108-112

[2]

Twist Confers Chemoresistance to Anthracyclines in Bladder Cancer through Upregulating P-Glycoprotein [J].

Chen, Yule ;

Li, Lei ;

Zeng, Jin ;

Wu, Kaijie ;

Zhou, Jiancheng ;

Guo, Peng ;

Zhang, Dong ;

Xue, Yan ;

Liang, Liang ;

Wang, Xinyang ;

Chang, Luke S. ;

He, Dalin .

CHEMOTHERAPY, 2012, 58 (04) :264-272

[3]

Multidrug resistance in cancer: Role of ATP-dependent transporters [J].

Gottesman, MM ;

Fojo, T ;

Bates, SE .

NATURE REVIEWS CANCER, 2002, 2 (01) :48-58

[4]

EXPRESSION OF MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN (MRP), MDR1 AND DNA TOPOISOMERASE-II IN HUMAN MULTIDRUG-RESISTANT BLADDER-CANCER CELL-LINES [J].

HASEGAWA, S ;

ABE, T ;

NAITO, S ;

KOTOH, S ;

KUMAZAWA, J ;

HIPFNER, DR ;

DEELEY, RG ;

COLE, SPC ;

KUWANO, M .

BRITISH JOURNAL OF CANCER, 1995, 71 (05) :907-913

[5]

MDR1 and ERCC1 Expression Predict Outcome of Patients with Locally Advanced Bladder Cancer Receiving Adjuvant Chemotherapy [J].

Hoffmann, Andreas-Claudius ;

Wild, Peter ;

Leicht, Christina ;

Bertz, Simone ;

Danenberg, Kathleen D. ;

Danenberg, Peter V. ;

Stoehr, Robert ;

Stoeckle, Michael ;

Lehmann, Jan ;

Schuler, Martin ;

Hartmann, Arndt .

NEOPLASIA, 2010, 12 (08) :628-636

[6]

Transcriptional activation of the MDR1 gene by UV irradiation - Role of NF-Y and Sp1 [J].

Hu, Z ;

Jin, SK ;

Scotto, KW .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (04) :2979-2985

[7]

Effects of sinomenine on proliferation and apoptosis in human lung cancer cell line NCI-H460 in vitro [J].

Jiang, Tingshu ;

Zhou, Liping ;

Zhang, Wenlu ;

Qu, Dan ;

Xu, Xiaoman ;

Yang, Ying ;

Li, Shengqi .

MOLECULAR MEDICINE REPORTS, 2010, 3 (01) :51-56

[8]

Bladder cancer [J].

Kaufman, Donald S. ;

Shipley, William U. ;

Feldman, Adam S. .

LANCET, 2009, 374 (9685) :239-249

[9]

Twist1-Mediated Adriamycin-Induced Epithelial-Mesenchymal Transition Relates to Multidrug Resistance and Invasive Potential in Breast Cancer Cells [J].

Li, Qing-Quan ;

Xu, Jing-Da ;

Wang, Wen-Juan ;

Cao, Xi-Xi ;

Chen, Qi ;

Tang, Feng ;

Chen, Zhong-Qing ;

Liu, Xiu-Ping ;

Xu, Zu-De .

CLINICAL CANCER RESEARCH, 2009, 15 (08) :2657-2665

[10]

Effect of sinomenine on gene expression of the IL-1β-activated human synovial sarcoma [J].

Li, X. J. ;

Yue, Patrick Y. K. ;

Ha, W. Y. ;

Wong, Daisy Y. L. ;

Tin, Mandy M. Y. ;

Wang, P. X. ;

Wong, Ricky N. S. ;

Liu, L. .

LIFE SCIENCES, 2006, 79 (07) :665-673