Stemar-13-ene synthase, a diterpene cyclase involved in the biosynthesis of the phytoalexin oryzalexin S in rice

被引:59
作者
Nemoto, T
Cho, EM
Okada, A
Okada, K
Otomo, K
Kanno, Y
Toyomasu, T
Mitsuhashi, W
Sassa, T
Minami, E
Shibuya, N
Nishiyama, M
Nojiri, H
Yamane, H
机构
[1] Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo 1138657, Japan
[2] Yamagata Univ, Dept Bioresource Engn, Yamagata 9978555, Japan
[3] Natl Inst Agrobiol Resources, Tsukuba, Ibaraki 3058602, Japan
[4] Meiji Univ, Dept Life Sci, Kanagawa 2160011, Japan
来源
FEBS LETTERS | 2004年 / 571卷 / 1-3期
关键词
phytoalexin; diterpene cyclase; stemar-13-ene; oryzalexin S; Oryza sativa L;
D O I
10.1016/j.febslet.2004.07.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In suspension-cultured rice cells, diterpenoid phytoalexins are produced in response to exogenously applied elicitors. We isolated a cDNA encoding a diterpene cyclase, OsDTC2, from suspension-cultured rice cells treated with a chitin elicitor. The OsDTC2 cDNA was overexpressed in Escherichia coli as a fusion protein with glutathione S-transferase, and the recombinant OsDTC2 was indicated to function as stemar-13-ene synthase that converted syn-copalyl diphosphate to stemar-13-ene, a putative diterpene hydrocarbon precursor of the phytoalexin oryzalexin S. The level of OsDTC2 mRNA in suspension-cultured rice cells began to increase 3 h after addition of the elicitor and reached the maximum after 8 h. The expression of OsDTC2 was also induced in UV-irradiated rice leaves. In addition, we indicated that stemar-13-ene accumulated in the chitin-elicited suspension-cultured rice cells and the UV-irradiated rice leaves. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
引用
收藏
页码:182 / 186
页数:5
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