Hydrogen-Rich Saline Protects Myocardium Against Ischemia/Reperfusion Injury in Rats

被引：187

作者：

Sun, Qiang ^{[1
]}

Kang, Zhimin ^{[1
]}

Cai, Jianmei ^{[1
]}

Liu, Wenwu ^{[1
]}

Liu, Yun ^{[1
]}

Zhang, John H. ^{[2
]}

Denoble, Petar J. ^{[3
]}

Tao, Hengyi ^{[1
]}

Sun, Xuejun ^{[1
]}

机构：

[1] Second Mil Med Univ, Dept Div Med, Fac Naval Med, Shanghai 200433, Peoples R China

[2] Loma Linda Univ, Dept Neurosurg, Loma Linda, CA 92350 USA

[3] Duke Univ Hlth Syst, Divers Alert Network, Durham, NC 27705 USA

来源：

EXPERIMENTAL BIOLOGY AND MEDICINE | 2009年 / 234卷 / 10期

基金：

中国国家自然科学基金;

关键词：

reperfusion; oxidative stress; hydrogen-rich saline; apoptosis; FREE-RADICAL GENERATION; REDUCES INFARCT SIZE; OXIDATIVE STRESS; ISCHEMIA; REPERFUSION; INHALATION; TRANSLATION; APOPTOSIS; THERAPY; MARKER;

D O I：

10.3181/0812-RM-349

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Protective effect of hydrogen (H-2) gas on cardiac ischemia-reperfusion (I/R) injury has been demonstrated previously. This study was designed to test the hypothesis that hydrogen-rich saline (saline saturated with molecular hydrogen), which is easy to use, induces cardioprotection against ischemia (30 min) and reperfusion (24 h) injury in rats. Adult male Sprague-Dawley rats underwent 30-min occlusion of the left anterior descending (LAD) coronary artery and 24-h reperfusion. Intraperitoneal injection of hydrogen-rich saline before reperfusion significantly decreased plasma and myocardium malondialdehyde (MDA) concentration, decreased cardiac cell apoptosis, and myocardial 8-hydroxydeoxyguanosine (8-OHdG) in area at risk zones (AAR), suppressed the activity of caspase-3, and reduced infarct size. The heart function parameters including left ventricular systolic pressure (LVSP), left ventricular diastolic pressure (LVDP), +(dP/dt)(max) and -(dP/dt)(max) were also significantly improved 24 h after reperfusion. It is concluded that hydrogen-rich saline is a novel, simple, safe, and effective method to attenuate myocardial I/R injury. Exp Biol Med 234:1212-1219, 2009

引用

页码：1212 / 1219

页数：8

共 32 条

[1] HYDROGENASE [J].

ADAMS, MWW ;

MORTENSON, LE ;

CHEN, JS .

BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 594 (2-3) :105-176

[2] Myocardial protection at a crossroads - The need for translation into clinical therapy [J].

Bolli, R ;

Becker, L ;

Gross, G ;

Mentzer, R ;

Balshaw, D ;

Lathrop, DA .

CIRCULATION RESEARCH, 2004, 95 (02) :125-134

[3] DEMONSTRATION OF FREE-RADICAL GENERATION IN STUNNED MYOCARDIUM OF INTACT DOGS WITH THE USE OF THE SPIN TRAP ALPHA-PHENYL N-TERT-BUTYL NITRONE [J].

BOLLI, R ;

PATEL, BS ;

JEROUDI, MO ;

LAI, EK ;

MCCAY, PB .

JOURNAL OF CLINICAL INVESTIGATION, 1988, 82 (02) :476-485

[4] MYOCARDIAL REPERFUSION - A DOUBLE-EDGED SWORD [J].

BRAUNWALD, E ;

KLONER, RA .

JOURNAL OF CLINICAL INVESTIGATION, 1985, 76 (05) :1713-1719

[5] Hydrogen inhalation ameliorates oxidative stress in transplantation induced intestinal graft injury [J].

Buchholz, B. M. ;

Kaczorowski, D. J. ;

Sugimoto, R. ;

Yang, R. ;

Wang, Y. ;

Billiar, T. R. ;

McCurry, K. R. ;

Bauer, A. J. ;

Nakao, A. .

AMERICAN JOURNAL OF TRANSPLANTATION, 2008, 8 (10) :2015-2024

[6] Direct evidence for S-nitrosation of mitochondrial complex I [J].

Burwell, LS ;

Nadtochiy, SM ;

Tompkins, AJ ;

Young, S ;

Brookes, PS .

BIOCHEMICAL JOURNAL, 2006, 394 :627-634

[7] Hydrogen therapy reduces apoptosis in neonatal hypoxia-ischemia rat model [J].

Cai, Jianmei ;

Kang, Zhimin ;

Liu, Wen Wu ;

Luo, Xu ;

Qiang, Sun ;

Zhang, John H. ;

Ohta, Shigeo ;

Sun, Xuejun ;

Xu, Weigang ;

Tao, Hengyi ;

Li, Runping .

NEUROSCIENCE LETTERS, 2008, 441 (02) :167-172

[8] Neuroprotective effects of hydrogen saline in neonatal hypoxia-ischemia rat model [J].

Cai, Jianmei ;

Kang, Zhimin ;

Liu, Kan ;

Liu, Wenwu ;

Li, RunPing ;

Zhang, John H. ;

Luo, Xu ;

Sun, Xuejun .

BRAIN RESEARCH, 2009, 1256 :129-137

[9] Reversible blockade of electron transport during ischemia protects mitochondria and decreases myocardial injury following reperfusion [J].

Chen, Qun ;

Moghaddas, Shadi ;

Hoppel, Charles L. ;

Lesnefsky, Edward J. .

JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2006, 319 (03) :1405-1412

[10] MALONDIALDEHYDE IS A BIOCHEMICAL MARKER OF PEROXIDATIVE DAMAGE IN THE ISOLATED REPERFUSED RAT-HEART [J].

DIPIERRO, D ;

TAVAZZI, B ;

LAZZARINO, G ;

GIARDINA, B .

MOLECULAR AND CELLULAR BIOCHEMISTRY, 1992, 116 (1-2) :193-196

← 1 2 3 4 →