Environmental DNA for wildlife biology and biodiversity monitoring

被引:859
作者
Bohmann, Kristine [1 ,2 ]
Evans, Alice [3 ]
Gilbert, M. Thomas P. [1 ,4 ]
Carvalho, Gary R. [3 ]
Creer, Simon [3 ]
Knapp, Michael [3 ]
Yu, Douglas W. [5 ,6 ]
de Bruyn, Mark [3 ]
机构
[1] Univ Copenhagen, Nat Hist Museum Denmark, Ctr GeoGenet, DK-1350 Copenhagen K, Denmark
[2] Univ Bristol, Sch Biol Sci, Bristol BS8 1UG, Avon, England
[3] Bangor Univ, Sch Biol Sci, Mol Ecol & Fisheries Genet Lab, Bangor LL57 2UW, Gwynedd, Wales
[4] Curtin Univ, Dept Environm & Agr, Trace & Environm DNA Lab, Perth, WA 6845, Australia
[5] Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650223, Yunnan, Peoples R China
[6] Univ E Anglia, Sch Biol Sci, Norwich NR4 7TJ, Norfolk, England
关键词
ANCIENT DNA; DIAGNOSTIC PCR; PREY DNA; DIET; AMPLIFICATION; SEDIMENTS; SAMPLES; DIRT; ICE; IDENTIFICATION;
D O I
10.1016/j.tree.2014.04.003
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Extraction and identification of DNA from an environmental sample has proven noteworthy recently in detecting and monitoring not only common species, but also those that are endangered, invasive, or elusive. Particular attributes of so-called environmental DNA (eDNA) analysis render it a potent tool for elucidating mechanistic insights in ecological and evolutionary processes. Foremost among these is an improved ability to explore ecosystem-level processes, the generation of quantitative indices for analyses of species, community diversity, and dynamics, and novel opportunities through the use of time-serial samples and unprecedented sensitivity for detecting rare or difficult-to-sample taxa. Although technical challenges remain, here we examine the current frontiers of eDNA, outline key aspects requiring improvement, and suggest future developments and innovations for research.
引用
收藏
页码:358 / 367
页数:10
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