Peptide bond formation by the industrial protease, neutrase, in organic media

被引：17

作者：

Clapes, P

Pera, E

Torres, JL

机构：

[1] Dept. of Peptide and Protein Chem., Ctro. de Invest. y Desarrollo, C.S.I.C., 08034-Barcelona

来源：

BIOTECHNOLOGY LETTERS | 1997年 / 19卷 / 10期

关键词：

D O I：

10.1023/A:1018407619672

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Neutrase, co-deposited with sorbitol on to polyamide, synthesised several N-alpha-protected dipeptide derivatives in acetonitrile with a buffer concentration of 4% (v/v). Reaction yields between 95-99% were obtained for all the dipeptides with the exception of Fmoc-Hyp-Leu-NH2 (77%) and Z-Val-Leu-NH2 (9%).

引用

页码：1023 / 1026

页数：4

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