Peptide bond formation by the industrial protease, neutrase, in organic media

被引:17
作者
Clapes, P
Pera, E
Torres, JL
机构
[1] Dept. of Peptide and Protein Chem., Ctro. de Invest. y Desarrollo, C.S.I.C., 08034-Barcelona
关键词
D O I
10.1023/A:1018407619672
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Neutrase, co-deposited with sorbitol on to polyamide, synthesised several N-alpha-protected dipeptide derivatives in acetonitrile with a buffer concentration of 4% (v/v). Reaction yields between 95-99% were obtained for all the dipeptides with the exception of Fmoc-Hyp-Leu-NH2 (77%) and Z-Val-Leu-NH2 (9%).
引用
收藏
页码:1023 / 1026
页数:4
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