Molecular basis of voltage-dependent delayed rectifier K+ channels in smooth muscle cells from rat tail artery

被引：25

作者：

Xu, CL ^{[1
]}

Tang, GH ^{[1
]}

Lu, YJ ^{[1
]}

Wang, R ^{[1
]}

机构：

[1] Univ Saskatchewan, Coll Med, Dept Physiol, Saskatoon, SK S7N 5E5, Canada

来源：

LIFE SCIENCES | 2000年 / 66卷 / 21期

基金：

英国医学研究理事会;

关键词：

Kv channels; smooth muscle cells; RT-PCR; Western blot;

D O I：

10.1016/S0024-3205(00)00529-4

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The molecular basis of voltage-dependent K+ (Kv) current in smooth muscle cells (SMCs) from rat tail artery was investigated by screening transcriptional expression of 15 Kv channel alpha-subunits and 3 Kv beta-subunits using RT-PCR technique. Among Kv genes that encode delayed rectifier Kv currents, mRNAs of Kv1.2, Kv1.3, Kv1.5, Kv2.1, Kv2.2, and Kv3.2 were expressed, but those of Kv1.1, Kv1.6, and Kv3.1 were not detected. The transient outward Kv current-encoding genes Kv1.4, Kv3.3, Kv3.4, Kv4.1- Kv4.3 as well as Kv beta 1, Kv beta 2, and Kv beta 3 were also expressed at mRNA level. Western blot study demonstrated the presence of Kv1.2, Kv1.3, Kv1.5, and Kv2.1, but not Kv3.2 proteins, in tail artery tissue. Immunocytochemistry study confirmed the presence of Kv1.2, Kv1.3, Kv1.5, and Kv2.1 channel proteins in primary cultured single SMCs. Our results represent the first systematic characterization of Kv gene expression in rat tail artery SMCs.

引用

页码：2023 / 2033

页数：11

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