Pluripotency of adult stem cells derived from human and rat pancreas

被引:52
作者
Kruse, C
Birth, M
Rohwedel, J
Assmuth, K
Goepel, A
Wedel, T
机构
[1] Med Univ Lubeck, Fraunhofer Inst Biomed Engn, Grp Cell Differentiat & Cell Technol, D-23538 Lubeck, Germany
[2] Med Univ Lubeck, Dept Surg, D-23538 Lubeck, Germany
[3] Med Univ Lubeck, Dept Med Mol Biol, D-23538 Lubeck, Germany
[4] Med Univ Lubeck, Dept Anat, D-23538 Lubeck, Germany
来源
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING | 2004年 / 79卷 / 07期
关键词
D O I
10.1007/s00339-004-2816-6
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Adult stem cells are undifferentiated cells found within fully developed tissues or organs of an adult individuum. Until recently, these cells have been considered to bear less self-renewal ability and differentiation potency compared to embryonic stem cells. In recent studies an undifferentiated cell type was found in primary cultures of isolated acini from exocrine pancreas termed pancreatic stellate cells. Here we show that pancreatic stellate-like cells have the capacity of extended self-renewal and are able to differentiate spontaneously into cell types of all three germ layers expressing markers for smooth muscle cells, neurons, glial cells, epithelial cells, chondrocytes and secretory cells (insulin, amylase). Differentiation and subsequent formation of three-dimensional cellular aggregates (organoid bodies) were induced by merely culturing pancreatic stellate-like cells in hanging drops. These cells were developed into stable, long-term, in vitro cultures of both primary undifferentiated cell lines as well as organoid cultures. Thus, evidence is given that cell lineages of endodermal, mesodermal, and ectodermal origin arise spontaneously from a single adult undifferentiated cell type. Based on the present findings it is assumed that pancreatic stellate-like cells are a new class of lineage uncommitted pluripotent adult stem cells with a remarkable self-renewal ability and differentiation potency. The data emphasize the versatility of adult stem cells and may lead to a reappraisal of their use for the treatment of inherited disorders or acquired degenerative diseases.
引用
收藏
页码:1617 / 1624
页数:8
相关论文
共 42 条
[1]   Insulinotropic hormone glucagon-like peptide-1 differentiation of human pancreatic islet-derived progenitor cells into insulin-producing cells [J].
Abraham, EJ ;
Leech, CA ;
Lin, JC ;
Zulewski, H ;
Habener, JF .
ENDOCRINOLOGY, 2002, 143 (08) :3152-3161
[2]   Periacinar stellate shaped cells in rat pancreas: identification, isolation, and culture [J].
Apte, MV ;
Haber, PS ;
Applegate, TL ;
Norton, ID ;
McCaughan, GW ;
Korsten, MA ;
Pirola, RC ;
Wilson, JS .
GUT, 1998, 43 (01) :128-133
[3]   Identification, culture, and characterization of pancreatic stellate cells in rats and humans [J].
Bachem, MG ;
Schneider, E ;
Gross, H ;
Weidenbach, H ;
Schmid, RM ;
Menke, A ;
Siech, M ;
Beger, H ;
Grünert, A ;
Adler, G .
GASTROENTEROLOGY, 1998, 115 (02) :421-432
[4]   In vitro cultivation of human islets from expanded ductal tissue [J].
Bonner-Weir, S ;
Taneja, M ;
Weir, GC ;
Tatarkiewicz, K ;
Song, KH ;
Sharma, A ;
O'Neil, JJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (14) :7999-8004
[5]   From marrow to brain: Expression of neuronal phenotypes in adult mice [J].
Brazelton, TR ;
Rossi, FMV ;
Keshet, GI ;
Blau, HM .
SCIENCE, 2000, 290 (5497) :1775-1779
[6]  
Elsasser H P, 1986, Pancreas, V1, P421, DOI 10.1097/00006676-198609000-00006
[7]   REPETITIVE CERULEIN-INDUCED PANCREATITIS AND PANCREATIC FIBROSIS IN THE RAT [J].
ELSASSER, HP ;
HAAKE, T ;
GRIMMIG, M ;
ADLER, G ;
KERN, HF .
PANCREAS, 1992, 7 (03) :385-390
[8]   In search of stem cell policy [J].
Frankel, MS .
SCIENCE, 2000, 287 (5457) :1397-1397
[9]  
GROSFILS K, 1993, RES COMMUN CHEM PATH, V79, P99
[10]   Absence of donor-derived keratinocyte stem cells in skin tissues cultured from patients after mobilized peripheral blood hematopoietic stem cell transplantation [J].
Hematti, P ;
Sloand, EM ;
Carvallo, CA ;
Albert, MR ;
Yee, CL ;
Fuehrer, MM ;
Blancato, JK ;
Kearns, WG ;
Barrett, JA ;
Childs, RW ;
Vogel, JC ;
Dunbar, CE .
EXPERIMENTAL HEMATOLOGY, 2002, 30 (08) :943-949