Soluble human MHC class I molecules induce soluble Fas ligand secretion and trigger apoptosis in activated CD8+ Fas (CD95)+ T lymphocytes

被引:94
作者
Puppo, F
Contini, P
Ghio, M
Brenci, S
Scudeletti, M
Filaci, G
Ferrone, S
Indiveri, F
机构
[1] Univ Genoa, Dept Internal Med, I-16132 Genoa, Italy
[2] Roswell Pk Canc Inst, Dept Immunol, Buffalo, NY 14263 USA
关键词
HLA; immunomodulation;
D O I
10.1093/intimm/12.2.195
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In the present study, we have evaluated the apoptotic effect of soluble human MHC class I (sHLA-I) antigens on CD8(+) T lymphocytes. sHLA-I antigens and beta(2)-microglobulin-free HLA class I heavy chains, isolated from serum, induced apoptosis on phytohemagglutinin-activated CD8(+) T lymphocytes in autologous and allogeneic combinations. The extent of CD8(+) T cell apoptosis depends on the degree of activation, time of incubation with sHLA-I antigens and amount of sHLA-I antigens added to the cultures. Apoptosis is induced by the interaction of Fas (CD95)(+) cells with soluble Fas ligand which is released following binding of sHLA-I antigens to CD8 molecules. These results suggest that sHLA-I antigens may regulate immune responses by inducing apoptosis in activated CD8(+) T cells.
引用
收藏
页码:195 / 203
页数:9
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