Ultrasensitive and quantitative analyses from combined separations-mass spectrometry for the characterization of proteomes

被引：126

作者：

Smith, RD ^{[1
]}

Shen, YF ^{[1
]}

Tang, KQ ^{[1
]}

机构：

[1] Pacific NW Natl Lab, Richland, WA 99352 USA

来源：

ACCOUNTS OF CHEMICAL RESEARCH | 2004年 / 37卷 / 04期

关键词：

D O I：

10.1021/ar0301330

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This article describes developments in fundamental and applied aspects of separations, electrospray ionization phenomena, and mass spectrometric instrumentation that are interrelated and important for making more effective and quantitative measurements, particularly for proteomics applications. The basis for better quantitation and ultrahigh sensitivity is highlighted for high-resolution capillary liquid chromatography separations that provide low nanoliter per minute flow rates to an electrospray ionization interface. The increased dynamic range of measurements and low zeptomole regime detection limits obtainable open new avenues for biological research.

引用

收藏

页码：269 / 278

页数：10

相关论文

共 49 条

[1]

Belov ME, 2000, J AM SOC MASS SPECTR, V11, P19, DOI 10.1016/S1044-0305(99)00121-X

[2] Zeptomole-sensitivity electrospray ionization - Fourier transform ion cyclotron resonance mass spectrometry of proteins [J].

Belov, ME ;

Gorshkov, MV ;

Udseth, HR ;

Anderson, GA ;

Smith, RD .

ANALYTICAL CHEMISTRY, 2000, 72 (10) :2271-2279

[3] Initial implementation, of external accumulation liquid chromatography/electrospray ionization Fourier transform ion cyclotron resonance with automated gain control [J].

Belov, ME ;

Rakov, VS ;

Nikolaev, EN ;

Goshe, MB ;

Anderson, GA ;

Smith, RD .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2003, 17 (07) :627-636

[4] Dynamic range expansion applied to mass spectrometry based on data-dependent selective ion ejection in capillary liquid chromatography Fourier transform ion cyclotron resonance for enhanced proteome characterization [J].

Belov, ME ;

Anderson, GA ;

Angell, NH ;

Shen, YF ;

Tolic, N ;

Udseth, HR ;

Smith, RD .

ANALYTICAL CHEMISTRY, 2001, 73 (21) :5052-5060

[5] Automated gain control and internal calibration with external ion accumulation capillary liquid chromatography-electrospray ionization-fourier transform ion cyclotron resonance [J].

Belov, ME ;

Zhang, R ;

Strittmatter, EF ;

Prior, DC ;

Tang, K ;

Smith, RD .

ANALYTICAL CHEMISTRY, 2003, 75 (16) :4195-4205

[6] Multiplex three-dimensional brain gene expression mapping in a mouse model of Parkinson's disease [J].

Brown, VM ;

Ossadtchi, A ;

Khan, AH ;

Yee, S ;

Lacan, G ;

Melega, WP ;

Cherry, SR ;

Leahy, RM ;

Smith, DJ .

GENOME RESEARCH, 2002, 12 (06) :868-884

[7] ''Colored'' noise waveforms and quadrupole excitation for the dynamic range expansion of Fourier transform ion cyclotron resonance mass spectrometry [J].

Bruce, JE ;

Anderson, GA ;

Smith, RD .

ANALYTICAL CHEMISTRY, 1996, 68 (03) :534-541

[8] Large molecule characterization based upon individual ion detection with electrospray ionization-FTICR mass spectrometry [J].

Bruce, JE ;

Anderson, GA ;

Udseth, HR ;

Smith, RD .

ANALYTICAL CHEMISTRY, 1998, 70 (03) :519-525

[9] MASS-SPECTROMETRY WITH ION SOURCES OPERATING AT ATMOSPHERIC-PRESSURE [J].

BRUINS, AP .

MASS SPECTROMETRY REVIEWS, 1991, 10 (01) :53-77

[10] Practical implications of some recent studies in electrospray ionization fundamentals [J].

Cech, NB ;

Enke, CG .

MASS SPECTROMETRY REVIEWS, 2001, 20 (06) :362-387

← 1 2 3 4 5 →