Cloning of a cDNA encoding a human homologue of CDC47, a member of the MCM family

被引：13

作者：

Kiyono, T ^{[1
]}

Fujita, M ^{[1
]}

Hayashi, Y ^{[1
]}

Ishibashi, M ^{[1
]}

机构：

[1] AICHI CANC CTR,RES INST,LAB VIRAL ONCOL,CHIKUSA KU,NAGOYA,AICHI 464,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1996年 / 1307卷 / 01期

关键词：

CDC47; MCM family; cloning; cDNA; (human);

D O I：

10.1016/0167-4781(96)00057-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A complementary DNA (cDNA) clone encoding a 719-amino acid (aa) protein was isolated, which has a 49% aa identity with budding yeast CDC47, a member of the MCM family. Antiserum raised against a C-terminal polypeptide bacterially produced from the cDNA clone detected a cellular protein about 80 kDa, which coincides with the size of the in vitro transcription/translation product of the cDNA. These results indicate that the cDNA covers the entire coding region of a human homologue of CDC47.

引用

页码：31 / 34

页数：4

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