Upregulation of glyceraldehyde-3-phosphate dehydrogenase mRNA in the spleen of tumor-bearing mice

被引：4

作者：

Aledo, JC ^{[1
]}

Segura, JA ^{[1
]}

Barbero, LG ^{[1
]}

Márquez, J ^{[1
]}

机构：

[1] Univ Malaga, Fac Ciencias, Dept Biol Mol & Bioquim, E-29071 Malaga, Spain

来源：

BIOCHIMIE | 1999年 / 81卷 / 12期

关键词：

Ehrlich ascites cell; splenocyte; GAPDH; immune response;

D O I：

10.1016/S0300-9084(99)00351-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence of tumor implantation on glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA levels and stability was determined in the spleen of tumor-bearing mice. While GAPDH mRNA levels were not altered in skeletal muscle, kidney and liver from tumor-bearing mice, tumor implantation led to a 5.6-fold increase in the levels of splenic GAPDH mRNA. An enhanced message stability was observed in splenocytes from tumor-bearing animals, suggesting the involvement of post-transcriptional mechanisms in the selective GAPDH mRNA accumulation after tumor implantation. The GAPDH activity/glycolytic flux ratio was 18.5 in the spleen of healthy mice. Therefore, the three-fold increase in the glycolytic flux observed after tumor implantation could hardly justify the necessity for the upregulation of GAPDH. (C) Society francaise de biochimie et biologie moleculaire/Editions scientifiques et medicales Elsevier SAS.

引用

页码：1109 / 1113

页数：5

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