Sample treatment for protein identification by mass spectrometry-based techniques

被引:53
作者
Lopez-Ferrer, D.
Canas, B.
Vazquez, J.
Lodeiro, C.
Rial-Otero, R.
Moura, I.
Capelo, J. L.
机构
[1] CSIC, Ctr Biol Mol Severo Ochoa, E-28049 Madrid, Spain
[2] Univ Complutense Madrid, Fac Quim, Dept Quim Anal, E-28040 Madrid, Spain
[3] Univ Nova Lisboa, Fac Ciencias & Tecnol, Dept Quim, REQUIMTE, P-2829516 Caparica, Portugal
关键词
biomarker; protein; rapid identification; sample treatment;
D O I
10.1016/j.trac.2006.05.015
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Rapid identification of proteins is of primary importance for the analytical community. Protein-biomarker discovery for medical diagnostics or pharmacological purposes is becoming one of the hottest research topics. Moreover, rapid identification of proteins can help unambiguous bacterial and virus detection. In addition, the fast identification of bacteria can be used to beat bioterrorism. As a consequence, new analytical methodologies have emerged recently with the aim of making protein analysis as fast and as confident as possible. In this article, we critically review the new trends in sample treatment for protein identification and comment on the prospects for the future in this promising analytical area. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:996 / 1005
页数:10
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