Chelidonic Acid and Its Derivatives from Saussurea Controversa: Isolation, Structural Elucidation and Influence on the Osteogenic Differentiation of Multipotent Mesenchymal Stromal Cells In Vitro

被引：16

作者：

Avdeeva, Elena ^{[1
]}

Shults, Elvira ^{[2
,3
]}

Rybalova, Tatyana ^{[3
,4
]}

Reshetov, Yaroslav ^{[1
]}

Porokhova, Ekaterina ^{[5
]}

Sukhodolo, Irina ^{[5
]}

Litvinova, Larisa ^{[6
]}

Shupletsova, Valeria ^{[6
]}

Khaziakhmatova, Olga ^{[6
]}

Khlusov, Igor ^{[5
,7
]}

Guryev, Artem ^{[1
]}

Belousov, Mikhail ^{[1
,7
]}

机构：

[1] Siberian State Med Univ, Dept Pharmaceut Anal, Tomsk 634050, Russia

[2] Novosibirsk Inst Organ Chem, Lab Med Chem, Siberian Branch, Novosibirsk 630090, Russia

[3] Novosibirsk State Univ, 2 Pirogova St, Novosibirsk 630090, Russia

[4] Novosibirsk Inst Organ Chem, Siberian Branch, Ctr Spectral Invest, Novosibirsk 630090, Russia

[5] Siberian State Med Univ, Dept Morphol & Gen Pathol, Tomsk 634050, Russia

[6] Immanuel Kant Baltic Fed Univ, Basic Lab Immunol & Cell Biotechnol, Kaliningrad 236041, Russia

[7] Tomsk Polytech Univ, Res Sch Chem & Appl Biomed Sci, Tomsk 634050, Russia

来源：

BIOMOLECULES | 2019年 / 9卷 / 05期

关键词：

Saussurea controversa DC (Asteraceae); chelidonic acid; 4-oxo-4H-pyran-2; 6-dicarboxylic acid esters; X-ray diffraction analysis; human multipotent mesenchymal stromal cells; INTERNATIONAL-SOCIETY; ADIPOSE-TISSUE; COMPLEXES;

D O I：

10.3390/biom9050189

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

4-oxo-4H-pyran-2.6-dicarboxylic acid (chelidonic acid, ChA) in the native state and in the complex with calcium [Ca(ChA)(H2O)(3)], named saucalchelin (CaChA), was isolated from the extract of Saussurea controversa leaves for the first time for the Asteraceae family. The structure of ChA was determined by NMR, MS and confirmed by X-ray analysis of its monomethyl ester, and CaChA was described by IR, ICP-MS, CHN analysis. The yield of ChA and CaChA was 45 mg/g and 70 mg/g of extract, respectively. The osteogenic activity of ChA, n-monobutyl ester of chelidonic acid, and CaChA has been studied in vitro in a 21-day culture of human adipose-derived multipotent mesenchymal stromal cells (hAMMSCs) in a standard nutrient medium without osteogenic supplements. CaChA significantly stimulated the growth of cell mass and differentiation of hAMMSCs into osteoblasts with subsequent mineralization of the culture and it may be a promising substance for accelerating bone tissue regeneration and engineering.

引用

页数：12

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