Identification of genes controlled by quorum sensing in Pseudomonas aeruginosa

被引:495
作者
Whiteley, M [1 ]
Lee, KM [1 ]
Greenberg, EP [1 ]
机构
[1] Univ Iowa, Dept Microbiol, Iowa City, IA 52242 USA
关键词
D O I
10.1073/pnas.96.24.13904
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Bacteria communicate with each other to coordinate expression of specific genes in a cell density-dependent fashion, a phenomenon called quorum sensing and response. Although we know that quorum sensing via acyl-homoserine lactone (HSL) signals controls expression of several virulence genes in the human pathogen Pseudomonas aeruginosa, the number and types of genes controlled by quorum sensing have not been studied systematically. We have constructed a library of random insertions in the chromosome of a P. aeruginosa acyl-HSL synthesis mutant by using a transposon containing a promoterless lacZ. This library was screened far acyl-HSL induction of lacZ. Thirty-nine quorum sensing-regulated genes were identified. The genes were organized into classes depending an the pattern of regulation. About half of the genes appear to be in seven operons, some seem organized in targe patches on the genome. Many of the quorum sensing-regulated genes code for putative virulence factors or production of secondary metabolites. Many of the genes identified showed a high level of induction by acyl-HSL signaling.
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页码:13904 / 13909
页数:6
相关论文
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