Specific polyclonal-based immunoassays for sulfathiazole

被引：48

作者：

Pastor-Navarro, N ^{[1
]}

García-Rover, C ^{[1
]}

Maquieira, A ^{[1
]}

Puchades, R ^{[1
]}

机构：

[1] Univ Politecn Valencia, Dept Quim, Valencia 46022, Spain

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2004年 / 379卷 / 7-8期

关键词：

antimicrobials; sulfonamides; sulfathiazole; ELISA; honey;

D O I：

10.1007/s00216-004-2683-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly sensitive and specific enzyme-linked immunosorbent assay has been developed for detection of sulfathiazole (STZ, 4-amino-N-thiazol-2-yl-benzene-sulfonamide). A set of haptens was synthesized in order to produce polyclonal antibodies against sulfonamides. Two ELISA formats (antibody-coated and conjugate-coated) were also investigated using all the serum/coating conjugate combinations that showed specific recognition. The developed ELISA succeeded in detection of STZ at concentrations as low as 0.03 ng mL(-1) over a measurable range of 0.12-6.71 ng mL(-1). Selectivity studies have demonstrated that other sulfonamides do not interfere significantly (< 10%) with analysis of STZ by this immunochemical technique. Analysis of spiked bee honey samples by the developed ELISA method showed recoveries were good. The selectivity and sensitivity (IC50 = 1.6 ng mL(-1)) make it a suitable screening method for determination of low levels of STZ in food samples.

引用

页码：1088 / 1099

页数：12

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