Replication, Pathogenesis and Transmission of Pandemic (H1N1) 2009 Virus in Non-Immune Pigs

被引：135

作者：

Brookes, Sharon M. ^{[1
]}

Nunez, Alejandro ^{[1
]}

Choudhury, Bhudipa ^{[1
,10
]}

Matrosovich, Mikhail ^{[7
]}

Essen, Stephen C. ^{[1
]}

Clifford, Derek ^{[1
]}

Slomka, Marek J. ^{[1
]}

Kuntz-Simon, Gaelle ^{[3
]}

Garcon, Fanny ^{[1
]}

Nash, Bethany ^{[1
]}

Hanna, Amanda ^{[1
]}

Heegaard, Peter M. H. ^{[6
]}

Queguiner, Stephane ^{[3
]}

Chiapponi, Chiara ^{[5
]}

Bublot, Michel ^{[8
]}

Maldonado Garcia, Jaime ^{[9
]}

Gardner, Rebecca ^{[1
]}

Foni, Emanuela ^{[5
]}

Loeffen, Willie ^{[4
]}

Larsen, Lars ^{[6
]}

Van Reeth, Kristien ^{[2
]}

Banks, Jill ^{[1
]}

Irvine, Richard M. ^{[1
]}

Brown, Ian H. ^{[1
]}

机构：

[1] Vet Labs Agcy Weybridge, EU, OIE, FAO Reference Lab Avian Influenza & Newcastle Dis, Addlestone, Surrey, England

[2] Univ Ghent, Merelbeke, Belgium

[3] Agence Francaise Securite Sanitaire Aliments, LERAPP, Unite Virol Immunol Porcines, Zoopole Les Croix, Ploufragan, France

[4] Cent Vet Inst Wageningen UR, Lelystad, Netherlands

[5] Ist Zooprofilatt Sperimentale Lombardia Emilia Ro, Sez Parma, Parma, Italy

[6] Tech Univ Denmark, Natl Vet Inst, Copenhagen, Denmark

[7] Univ Marburg, Inst Virol, D-3550 Marburg, Germany

[8] R&D, SAS, Lyon, France

[9] Labs HIPRA, Amer Girona, Spain

[10] World Org Anim Hlth, OFFLU, Paris, France

来源：

PLOS ONE | 2010年 / 5卷 / 02期

关键词：

AVIAN INFLUENZA-VIRUSES; ACUTE-PHASE PROTEIN; RECEPTOR-BINDING PROPERTIES; C-REACTIVE PROTEIN; INTERSPECIES TRANSMISSION; EXPERIMENTAL-INFECTION; MDCK CELLS; HEMAGGLUTININ; STRAINS; FERRETS;

D O I：

10.1371/journal.pone.0009068

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The declaration of the human influenza A pandemic (H1N1) 2009 (H1N1/09) raised important questions, including origin and host range [1,2]. Two of the three pandemics in the last century resulted in the spread of virus to pigs (H1N1, 1918; H3N2, 1968) with subsequent independent establishment and evolution within swine worldwide [3]. A key public and veterinary health consideration in the context of the evolving pandemic is whether the H1N1/09 virus could become established in pig populations [4]. We performed an infection and transmission study in pigs with A/California/07/09. In combination, clinical, pathological, modified influenza A matrix gene real time RT-PCR and viral genomic analyses have shown that infection results in the induction of clinical signs, viral pathogenesis restricted to the respiratory tract, infection dynamics consistent with endemic strains of influenza A in pigs, virus transmissibility between pigs and virus-host adaptation events. Our results demonstrate that extant H1N1/09 is fully capable of becoming established in global pig populations. We also show the roles of viral receptor specificity in both transmission and tissue tropism. Remarkably, following direct inoculation of pigs with virus quasispecies differing by amino acid substitutions in the haemagglutinin receptor-binding site, only virus with aspartic acid at position 225 (225D) was detected in nasal secretions of contact infected pigs. In contrast, in lower respiratory tract samples from directly inoculated pigs, with clearly demonstrable pulmonary pathology, there was apparent selection of a virus variant with glycine (225G). These findings provide potential clues to the existence and biological significance of viral receptor-binding variants with 225D and 225G during the 1918 pandemic [5].

引用

页数：9

共 35 条

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