Prostaglandin E2 stimulates AP-1-mediated CD14 expression in mouse macrophages via cyclic AMP-dependent protein kinase A

PGs play a functional role in the early stage of Gram-negative bacterial infections, because this prostanoid is produced rapidly by epithelial cells after a bacterial infection. CD14, one of the LPS receptors, is a key molecule in triggering the response to bacterial LPS in association with a Toll-like molecule. Therefore, in this study, we investigated the effect of PG on CD14 expression in mouse macrophages. PGE(1), PGE(2), and PGA(1) among the PGs tested strongly stimulated the expression of the CD14 gene in the cells, The stimulatory action also was observed by Western blot analysis. cAMP elevating agents stimulated expression of CD14 gene as well, Protein kinase A inhibitor, N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89), but not protein kinase C inhibitor 3-{1-[3-(dimethylamino)propyl]-1H-indol-3-yl}-4-(1H-indol-3-yl)-1H-pyrrole-2,5-dione (GF109203X), abolished the stimulated expression of CD14, A run-on assay showed that PGE(2) stimulated the CD14 gene expression at the transcriptional level via protein kinase A. PGE(2) also stimulated activation of AP-1, a heterodimer of c-Jun and c-Fos, because the prostanoid increased specific binding of nuclear proteins to the AP-1 consensus sequence and stimulated AP-1-promoted luciferase activity, PGE(2)-stimulated expression of CD14 was inhibited by antisense c-fos and c-jun oligonucleotides, but not by their sense oligonucleotides, Finally, PGE(2) pretreatment synergistically stimulated LPS-induced expression of IL-1 beta and IL-6 genes in mouse macrophages, Therefore, the present study demonstrates that PGE(2) has the ability to stimulate AP-1-mediated expression of CD14 in mouse macrophages via cAMP-dependent protein kinase A.