Transferability of microsatellite markers from Eucalyptus, spp. to Eugenia dysenterica (Myrtaceae family)

被引:35
作者
Zucchi, MI
Brondani, RPV
Pinheiro, JB
Brondani, C
Vencovsky, R
机构
[1] Univ Sao Paulo, ESALQ, Dept Genet, BR-13400970 Piracicaba, SP, Brazil
[2] Univ Fed Goias, Escola Agron, BR-74001970 Goiania, Go, Brazil
来源
MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 04期
关键词
cerrado; Eugenia dysenterica; gene flow; microsatellite; Myrtaceae family; transferability;
D O I
10.1046/j.1471-8286.2002.00297.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cagaita (Eugenia dysenterica) is a widespread plant found in the Brazilian cerrado. Its fruit is used for popular consumption and for industrial purposes. A battery of 346 Primer pairs developed for Eucalyptus spp. was tested on cagaita. Only 10 primer pairs were found to be transferable between the two species. Using a polyacrilamide gel, an average of 10.4 alleles per locus was detected, in a sample of 116 individuals from 10 natural populations of cagaita. Seven polymorphic loci allowed estimation of genetic parameters, including expected average heterozigosity H-E = 0.442, diversity among populations, R-ST = 0.268 and gene flow Nm = 0.680.
引用
收藏
页码:512 / 513
页数:2
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