A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding

被引：65

作者：

Liu, Li ^{[1
,2
]}

Wang, Aili ^{[1
,3
]}

Appels, Rudi ^{[6
]}

Ma, Junhong ^{[1
]}

Xia, Xianchun ^{[2
]}

Lan, Ping ^{[1
]}

He, Zhonghu ^{[2
,4
]}

Bekes, Frank ^{[5
]}

Yan, Yueming ^{[3
]}

Ma, Wujun ^{[1
]}

机构：

[1] Murdoch Univ, Western Australia Dept Agr & Food, SABC, Mol Plant Breeding CRC, Perth, WA 6150, Australia

[2] Chinese Acad Agr Sci, Inst Crop Sci, Natl Wheat Improvement Ctr, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

[3] Capital Normal Univ, Coll Life Sci, Key Lab Genet & Biotechnol, Beijing 100037, Peoples R China

[4] Chinese Acad Agr Sci, CIMMYT, China Off, Beijing 100081, Peoples R China

[5] CSIRO Plant Ind, Canberra, ACT, Australia

[6] Murdoch Univ, Ctr Comparat Genom, Perth, WA 6150, Australia

来源：

JOURNAL OF CEREAL SCIENCE | 2009年 / 50卷 / 02期

关键词：

Triticum aestivum L; Quality; SDS-PAGE; MALDI-TOF-MS; HMW-GS; ASSISTED-LASER-DESORPTION/IONIZATION; IONIZATION MASS-SPECTROMETRY; TRITICUM-AESTIVUM L; BREAD-MAKING QUALITY; ALLELIC VARIATION; STORAGE PROTEINS; GROUP-1; CHROMOSOMES; DOUGH PROPERTIES; COMMON WHEAT; GLU-3; LOCI;

D O I：

10.1016/j.jcs.2009.05.006

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

High molecular weight glutenin subunits play an important role in determining wheat dough quality as they confer visco-elastic properties to the dough required for mixing and baking performance. In this work, a collection of 103 genotypes of common wheat from 12 countries was used to analyse the composition of HMW-GS by SDS-PAGE and MALDI-TOF-MS. Results indicated that MALDI-TOF technology is suitable for analyzing most HMW-GS alleles. The allelic diversity at Glu-B1 locus include subunits 6+8b*, 7, 7+8, 7+8a*, 7b*+8, 7(OE), 7(OE)+8, 7(OE)+8a*, 7(OE)+8b*, 7+9, 13+16, 14+15, 17+18 and 20. The rapid identification of HMW-GS capability of MALDI-TOF-MS is discussed in relation to its value for screening lines in wheat breeding programs, especially in discriminating subunits 7(OE), 8a* and 8b* associated with superior quality. A new glutenin subunit 7b*+8 was found in Japanese germplasm Eshimashinriki. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：295 / 301

页数：7

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