Identification of serum microRNAs as diagnostic and prognostic biomarkers for acute pancreatitis

被引：69

作者：

Liu, Pi ^{[1
]}

Xia, Liang ^{[1
]}

Zhang, Wei-long ^{[2
]}

Ke, Hua-jing ^{[1
]}

Su, Tao ^{[1
]}

Deng, Li-bing ^{[2
]}

Chen, You-xiang ^{[1
]}

Lv, Nong-hua ^{[1
]}

机构：

[1] Nanchang Univ, Affiliated Hosp 1, Dept Gastroenterol, Nanchang 330006, Peoples R China

[2] Nanchang Univ, Nanchang 330031, Peoples R China

来源：

PANCREATOLOGY | 2014年 / 14卷 / 03期

基金：

中国国家自然科学基金;

关键词：

microRNA; Acute pancreatitis; Early diagnosis; Prognosis; Biomarker; Prediction; ISLET-SPECIFIC MICRORNAS; EXPRESSION PROFILE; SEVERITY; TARGETS; CELLS; PROCALCITONIN; PREDICTION; MORTALITY; MIR-10A; KINASE;

D O I：

10.1016/j.pan.2014.03.019

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background/objectives: To identify serum microRNA (miRNA) as diagnostic and prognostic biomarkers for acute pancreatitis (AP). Materials and methods: Sera microRNA expression was profiled from 12 AP patients with varying disease severity and three healthy controls. Differentially expressed miRNAs were validated in a larger cohort of patients and controls. The diagnostic and prognostic potentials of differentially expressed miRNAs were evaluated using receiver operating characteristic (ROC) curve analysis and compared to that of classic prognostic markers for AP. Results: miRNA microarray analyses identified 205 differentially expressed miRNAs between sera from AP patients and that from controls. Nine miRNAs were differentially expressed between severe and mild AP patients. Further validation confirmed the down-regulation of miR-92b, miR-10a, and miR-7 in AP patients, and ROC analysis revealed that these miRNAs can differentiate AP from health cases. Furthermore, the serum miR-551b-5p level was significantly higher in patients with disease complications or a low plasma calcium level. ROC analysis showed that the serum miR-551b-5p level can distinguish between severe and mild AP. Conclusion: The expressions of miR-92b, miR-10a, and miR-7 in AP might be used for the early diagnosis of AP and miR-551b-5p may be used for predicting AP severity. Copyright (C) 2014, IAP and EPC. Published by Elsevier India, a division of Reed Elsevier India Pvt. Ltd. All rights reserved.

引用

页码：159 / 166

页数：8

共 41 条

[11] miRNA expression profile in primary gastric cancers and paired lymplh node metastases indicates that miR-10a plays a role in metastasis from primary gastric cancer to lymph nodes [J].

Chen, Weidong ;

Tang, Zhaoqing ;

Sun, Yihong ;

Zhang, Youyou ;

Wang, Xuefei ;

Shen, Zhenbin ;

Liu, Fenglin ;

Qin, Xinyu .

EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2012, 3 (02) :351-356

[12] miR-92b regulates Mef2 levels through a negative-feedback circuit during Drosophila muscle development [J].

Chen, Zhimin ;

Liang, Shanshan ;

Zhao, Ying ;

Han, Zhe .

DEVELOPMENT, 2012, 139 (19) :3543-3552

[13] MicroRNA-92b regulates expression of the oligopeptide transporter PepT1 in intestinal epithelial cells [J].

Dalmasso, Guillaume ;

Hang Thi Thu Nguyen ;

Yan, Yutao ;

Laroui, Hamed ;

Charania, Moiz A. ;

Obertone, Tracy S. ;

Sitaraman, Shanthi V. ;

Merlin, Didier .

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2011, 300 (01) :G52-G59

[14] VDAC1 selectively transfers apoptotic Ca2+ signals to mitochondria [J].

De Stefani, D. ;

Bononi, A. ;

Romagnoli, A. ;

Messina, A. ;

De Pinto, V. ;

Pinton, P. ;

Rizzuto, R. .

CELL DEATH AND DIFFERENTIATION, 2012, 19 (02) :267-273

[15]

Dervenis C, 1999, INT J PANCREATOL, V25, P195

[16] Prediction of Mortality in Acute Pancreatitis: A Systematic Review of the Published Evidence [J].

Gravante, G. ;

Garcea, G. ;

Ong, S. L. ;

Metcalfe, M. S. ;

Berry, D. P. ;

Lloyd, D. M. ;

Dennison, A. R. .

PANCREATOLOGY, 2009, 9 (05) :601-614

[17] miRBase: microRNA sequences, targets and gene nomenclature [J].

Griffiths-Jones, Sam ;

Grocock, Russell J. ;

van Dongen, Stijn ;

Bateman, Alex ;

Enright, Anton J. .

NUCLEIC ACIDS RESEARCH, 2006, 34 :D140-D144

[18] Acute pancreatitis [J].

Harper, Simon J. F. ;

Cheslyn-Curtis, Sarah .

ANNALS OF CLINICAL BIOCHEMISTRY, 2011, 48 :23-37

[19] MicroRNA 10a Marks Regulatory T Cells [J].

Jeker, Lukas T. ;

Zhou, Xuyu ;

Gershberg, Kseniya ;

de Kouchkovsky, Dimitri ;

Morar, Malika M. ;

Stadthagen, Gustavo ;

Lund, Anders H. ;

Bluestone, Jeffrey A. .

PLOS ONE, 2012, 7 (05)

[20]

Joglekar MV, 2011, INDIAN J EXP BIOL, V49, P401

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