Four-color, enzyme-free interrogation of DNA sequences with chemically activated, 3′-fluorophore-labeled nucleotides

被引：26

作者：

Griesang, Niels ^{[1
]}

Giessler, Kerstin ^{[1
]}

Lommel, Tanja ^{[1
]}

Richert, Clemens ^{[1
]}

机构：

[1] Univ Karlsruhe, Inst Organ Chem, D-76131 Karlsruhe, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 37期

关键词：

DNA recognition; DNA replication; oligonucleotides; primer extension; single-nucleotide polymorphism;

D O I：

10.1002/anie.200600804

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Color reveals base: The enzymatic template-directed elongation of primers with fluorophore-labeled nucleoside triphosphates is the most frequently used reaction for determining DNA sequences. Primer extension followed by optical read out can now be achieved without enzymes. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：6144 / 6148

页数：5

共 40 条

[1] NMR studies of DNA duplexes singly cross-linked by different synthetic linkers [J].

Altmann, S ;

Labhardt, AM ;

Bur, D ;

Lehmann, C ;

Bannwarth, W ;

Billeter, M ;

Wuthrich, K ;

Leupin, W .

NUCLEIC ACIDS RESEARCH, 1995, 23 (23) :4827-4835

[2] The $1,000 genome [J].

Arnaud, CH .

CHEMICAL & ENGINEERING NEWS, 2005, 83 (51) :60-61

[3] Synthesis of oligonucleotides with 3′-terminal 5-(3-acylamidopropargyl)-3′-amino-2′,3′-dideoxyuridine residues and their reactivity in single-nucleotide steps of chemical replication [J].

Baumhof, P ;

Griesang, N ;

Bächle, M ;

Richert, C .

JOURNAL OF ORGANIC CHEMISTRY, 2006, 71 (03) :1060-1067

[4] Non-enzymatic transcription of an IsoG•IsoC base pair [J].

Chaput, JC ;

Switzer, C .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (51) :12866-12867

[5] Synthesis of oligonucleotides with a 2′-cap at the 3′-terminus via reversed phosphoramidites [J].

Connors, WH ;

Narayanan, S ;

Kryatova, OP ;

Richert, C .

ORGANIC LETTERS, 2003, 5 (03) :247-250

[6] 5′-Tethered stilbene derivatives as fidelity- and affinity-enhancing modulators of DNA duplex stability [J].

Dogan, Z ;

Paulini, R ;

Stütz, JAR ;

Narayanan, S ;

Richert, C .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (15) :4762-4763

[7]

Dombi KL, 2002, SYNTHESIS-STUTTGART, P816

[8] Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 Å resolution [J].

Doublié, S ;

Tabor, S ;

Long, AM ;

Richardson, CC ;

Ellenberger, T .

NATURE, 1998, 391 (6664) :251-258

[9] Single-nucleotide-specific PNA-peptide ligation on synthetic and PCR DNA templates [J].

Ficht, S ;

Mattes, A ;

Seitz, O .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (32) :9970-9981

[10] Linker-free fluorophore-labeled oligonucleotides:: Synthesis of 3′-fluoresceinylthymidine building blocks and their coupling reactions [J].

Griesang, N ;

Kervio, E ;

Richert, C .

SYNTHESIS-STUTTGART, 2005, (14) :2327-2334

← 1 2 3 4 →