2,5-Diketopiperazines Produced by Bacillus pumilus During Bacteriolysis of Arthrobacter citreus

被引：16

作者：

Brack, Christiane ^{[1
]}

Mikolasch, Annett ^{[1
]}

Schauer, Frieder ^{[1
]}

机构：

[1] Ernst Moritz Arndt Univ Greifswald, Inst Microbiol, Dept Appl Microbiol, D-17487 Greifswald, Germany

来源：

MARINE BIOTECHNOLOGY | 2014年 / 16卷 / 04期

关键词：

Cyclic peptides; Bacterivory; Intraguild predation; Lysobacter; CYCLIC DIPEPTIDES; NATURAL-PRODUCTS; MARINE-BACTERIA; CROSS-TALK; DIKETOPIPERAZINES; IDENTIFICATION; ANTIBIOTICS; LYSOBACTER; PEPTIDES;

D O I：

10.1007/s10126-014-9559-y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report the detection by gas chromatography/mass spectrometry and liquid chromatography/mass spectrometry analyses of the secreted 2,5-diketopiperazines (DKPs) cyclo(-Ala-Pro), cyclo(-Gly-Pro), cyclo(-Val-Pro), cyclo(-Ile-Pro), cyclo(-Leu-Pro), cyclo(-Pro-Pro), cyclo(-HyP-Pro), cyclo(-Met-Pro), and cyclo(-Phe-Pro) produced by Bacillus pumilus. The study focuses on a marine isolate and a laboratory test strain of B. pumilus with capabilities to lyse pregrown living cell lawns of different bacterial species, among them Arthrobacter citreus. Chromatographic methods were used to analyze induced bioactive compounds. At least 13 different DKPs are produced by B. pumilus. Both strains respond with an increased production of the DKPs cyclo(-Gly-Pro), cyclo(-Ala-Pro), and cyclo(-Val-Pro) to the presence of pasteurized A. citreus cells after 4 h in a nutrient-poor liquid medium. In agar diffusion assays, these DKPs did not cause lysis zones in living cell lawns, but they did inhibit further growth of several pregrown test bacteria in microplates even at concentrations as low as 1 mu g ml(-1). Antibiotic substances produced by B. pumilus after 20 h of cultivation in a special lysis medium showed lytic activity in cell-free extracts of B. pumilus culture supernatants.

引用

页码：385 / 395

页数：11

共 30 条

[1] Deciphering the hunting strategy of a bacterial wolfpack [J].

Berleman, James E. ;

Kirby, John R. .

FEMS MICROBIOLOGY REVIEWS, 2009, 33 (05) :942-957

[2] The Cyclic Dipeptide Cyclo(Phe-Pro) Inhibits Cholera Toxin and Toxin-Coregulated Pilus Production in O1 El Tor Vibrio cholerae [J].

Bina, Xiaowen R. ;

Bina, James E. .

JOURNAL OF BACTERIOLOGY, 2010, 192 (14) :3829-3832

[3] Bacteriolytic Bacillus species isolated from brackish waters of the Southern Baltic Sea [J].

Brack, Christiane ;

Mikolasch, Annett ;

Pukall, Ruediger ;

Schumann, Peter ;

Koester, Marion ;

Schauer, Frieder .

MARINE BIOLOGY, 2013, 160 (10) :2699-2709

[4] Microbial antagonism: a neglected avenue of natural products research [J].

Burgess, JG ;

Jordan, EM ;

Bregu, M ;

Mearns-Spragg, A ;

Boyd, KG .

JOURNAL OF BIOTECHNOLOGY, 1999, 70 (1-3) :27-32

[5]

de Carvalho MP, 2012, CURR MED CHEM, V19, P3564

[6] Marine bacteria associated with sponge as source of cyclic peptides [J].

De Rosa, S ;

Mitova, M ;

Tommonaro, G .

BIOMOLECULAR ENGINEERING, 2003, 20 (4-6) :311-316

[7] Plant growth-promoting Pseudomonas putida WCS358 produces and secretes four cyclic dipeptides:: Cross-talk with quorum sensing bacterial sensors [J].

Degrassi, G ;

Aguilar, C ;

Bosco, M ;

Zahariev, S ;

Pongor, S ;

Venturi, V .

CURRENT MICROBIOLOGY, 2002, 45 (04) :250-254

[8]

Fiolka MJ, 2004, FOLIA BIOL-KRAKOW, V52, P233

[9] Diketopiperazines in peptide and combinatorial chemistry [J].

Fischer, PM .

JOURNAL OF PEPTIDE SCIENCE, 2003, 9 (01) :9-35

[10] Production of antifungal compounds by Lysobacter enzymogenes isolate 3.1T8 under different conditions in relation to its efficacy as a biocontrol agent of Pythium aphanidermatum in cucumber [J].

Folman, LB ;

De Klein, MJEM ;

Postma, J ;

van Veen, JA .

BIOLOGICAL CONTROL, 2004, 31 (02) :145-154

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