Porphyromonas gingivalis genes involved in community development with Streptococcus gordonii

被引:65
作者
Simionato, M. Regina
Tucker, Chelsea M.
Kuboniwa, Masae
Lamont, Gwyneth
Demuth, Donald R.
Tribble, Gena D. [1 ]
Lamont, Richard J.
机构
[1] Univ Florida, Dept Oral Biol, Coll Dent, Gainesville, FL 32610 USA
[2] Univ Sao Paulo, Dept Microbiol, Inst Biomed Sci, Sao Paulo, Brazil
[3] Osaka Univ, Dept Prevent Dent, Grad Sch Dent, Suita, Osaka 5650871, Japan
[4] Univ Louisville, Dept Periodontol & Dent Hyg, Louisville, KY 40292 USA
关键词
D O I
10.1128/IAI.00639-06
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Porphyromonas gingivalis, one of the causative agents of adult periodontitis, develops biofilm microcolonies on substrata of Streptococcus gordonii but not on Streptococcus mutans. P. gingivalis genome microarrays were used to identify genes differentially regulated during accretion of P. gingivalis in heterotypic biofilms with S. gordonii. Thirty-three genes showed up- or downregulation by array analysis, and differential expression was confirmed by quantitative reverse transcription-PCR. The functions of the regulated genes were predominantly related to metabolism and energy production. In addition, many of the genes have no current known function. The roles of two upregulated genes,ftsH (PG0047) encoding an ATP-dependent zinc metallopepticlase and ptpA (PG1641) encoding a putative tyrosine phosphatase, were investigated further by mutational analysis. Strains with mutations in these genes developed more abundant biofilms with S. gordonii than the parental strain developed. ftsH and ptpA may thus participate in a regulatory network that constrains P. gingivalis accumulation in heterotypic biofilms. This study provided a global analysis of P. gingivalis transcriptional responses in an oral microbial community and also provided insight into the regulation of heterotypic biofilm development.
引用
收藏
页码:6419 / 6428
页数:10
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