HHV-6 infects human aortic and heart microvascular endothelial cells, increasing their ability to secrete proinflammatory chemokines

被引:67
作者
Caruso, A
Rotola, A
Comar, M
Favilli, F
Galvan, M
Tosetti, M
Campello, C
Caselli, E
Alessandri, G
Grassi, M
Garrafa, E
Cassai, E
Di Luca, D
机构
[1] Univ Ferrara, Dept Expt & Diagnost Med, Microbiol Sect, I-44100 Ferrara, Italy
[2] Univ Brescia, Inst Microbiol, Brescia, Italy
[3] Univ Trieste, UCO Hyg & Prevent Med, Burlo Garofolo, Italy
[4] IRCCS, Burlo Garofolo, Italy
关键词
herpesvirus; endothelium; inflammation; MCP-1; IL-8;
D O I
10.1002/jmv.10133
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Endothelial cells are important targets for herpesvirus infection. To evaluate the biological effects of human herpesvirus-6 (HHV-6) infection, adult heart microvascular and aortic endothelial cells were examined for in vitro susceptibility to HHV-6 and for the alterations induced by viral infection on the production of monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8). Analysis by reverse transcription-polymerase chain reaction and by in situ polymerase chain reaction showed that HHV-6 replicates in endothelium in the absence of cytopathic effects, and that viral sequences were present in 20% umbilical vein and in 10% aortic and 1% microvascular endothelium. HHV-6 infection upregulated the production of MCP-1 and IL-8, with differences observed between aortic and microvascular endothelium. These findings demonstrate that endothelial cells represent a potential reservoir for HHV-6 infection, and the altered pattern of chemokine production can lead to attraction of immunocompetent cells and to the development of inflammatory processes. (C) 2002 Wiley-Liss, Inc.
引用
收藏
页码:528 / 533
页数:6
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