Analysis of alternative splicing with microarrays: successes and challenges

被引：50

作者：

Lee, C ^{[1
]}

Roy, M ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Inst Mol Biol, Ctr Genom & Proteom, Dept Chem & Biochem, Los Angeles, CA 90095 USA

来源：

GENOME BIOLOGY | 2004年 / 5卷 / 07期

关键词：

D O I：

10.1186/gb-2004-5-7-231

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recently, DNA microarrays have emerged as potentially powerful tools for analyzing alternative splicing. We briefly review the latest results in this field and highlight the current challenges that they have revealed.

引用

页数：4

共 27 条

[1] EST comparison indicates 38% of human mRNAs contain possible alternative splice forms [J].

Brett, D ;

Hanke, J ;

Lehmann, G ;

Haase, S ;

Delbrück, S ;

Krueger, S ;

Reich, J ;

Bork, P .

FEBS LETTERS, 2000, 474 (01) :83-86

[2] The use and analysis of microarray data [J].

Butte, A .

NATURE REVIEWS DRUG DISCOVERY, 2002, 1 (12) :951-960

[3] Optimization of oligonucleotide arrays and RNA amplification protocols for analysis of transcript structure and alternative splicing [J].

Castle, J ;

Garrett-Engele, P ;

Armour, CD ;

Duenwald, SJ ;

Loerch, PM ;

Meyer, MR ;

Schadt, EE ;

Stoughton, R ;

Parrish, ML ;

Shoemaker, DD ;

Johnson, JM .

GENOME BIOLOGY, 2003, 4 (10)

[4] Genomewide analysis of mRNA processing in yeast using splicing-specific microarrays [J].

Clark, TA ;

Sugnet, CW ;

Ares, M .

SCIENCE, 2002, 296 (5569) :907-910

[5] ISIS, the intron information system, reveals the high frequency of alternative splicing in the human genome [J].

Croft, L ;

Schandorff, S ;

Clark, F ;

Burrage, K ;

Arctander, P ;

Mattick, JS .

NATURE GENETICS, 2000, 24 (04) :340-341

[6]

Heber Steffen, 2002, Bioinformatics, V18 Suppl 1, pS181

[7] Predicting splice variant from DNA chip expression data [J].

Hu, GK ;

Madore, SJ ;

Moldover, B ;

Jatkoe, T ;

Balaban, D ;

Thomas, J ;

Wang, YX .

GENOME RESEARCH, 2001, 11 (07) :1237-1245

[8] Alternative splicing and programmed cell death [J].

Jiang, ZH ;

Wu, JY .

PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE, 1999, 220 (02) :64-72

[9] Genome-wide survey of human alternative pre-mRNA splicing with exon junction microarrays [J].

Johnson, JM ;

Castle, J ;

Garrett-Engele, P ;

Kan, ZY ;

Loerch, PM ;

Armour, CD ;

Santos, R ;

Schadt, EE ;

Stoughton, R ;

Shoemaker, DD .

SCIENCE, 2003, 302 (5653) :2141-2144

[10] Novel RNAs identified from an in-depth analysis of the transcriptome of human chromosomes 21 and 22 [J].

Kampa, D ;

Cheng, J ;

Kapranov, P ;

Yamanaka, M ;

Brubaker, S ;

Cawley, S ;

Drenkow, J ;

Piccolboni, A ;

Bekiranov, S ;

Helt, G ;

Tammana, H ;

Gingeras, TR .

GENOME RESEARCH, 2004, 14 (03) :331-342

← 1 2 3 →