The mouse Pol I terminator is more efficient than the hepatitis delta virus ribozyme in generating influenza-virus-like RNAs with precise 3' ends in a plasmid-only-based virus rescue system

被引：11

作者：

Feng, Liqiang ^{[1
,2
]}

Li, Feng ^{[1
]}

Zheng, Xuehua ^{[1
]}

Pan, Weiqi ^{[1
]}

Zhou, Kai ^{[2
,3
]}

Liu, Yichu ^{[1
]}

He, Hongxuan ^{[3
]}

Chen, Ling ^{[1
]}

机构：

[1] Chinese Acad Sci, Ctr Vaccines & Biotherapeut, Guangzhou Inst Biomed & Hlth, Guangzhou 510663, Guangdong, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

[3] Chinese Acad Sci, Key Lab Anim Ecol & Conservat Biol, Natl Res Ctr Wildlife Born Dis, Inst Zool, Beijing 100101, Peoples R China

来源：

ARCHIVES OF VIROLOGY | 2009年 / 154卷 / 07期

关键词：

REVERSE GENETICS SYSTEM; A VIRUS; PACKAGING SIGNALS; SEGMENTS; VIRIONS; REGIONS; GENES; VRNA; DNA;

D O I：

10.1007/s00705-009-0422-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Reverse genetics systems for generating recombinant influenza viruses are based on two different mechanisms for obtaining the 3' end of the viral RNA: one uses the self-cleaving hepatitis delta virus ribozyme (HDVR), and the other uses the murine RNA polymerase I (Pol I) terminator. In this study, we employed EGFP and Renilla luciferase reporter constructs to compare the efficiency of both methods. Our results indicate that the murine Pol I terminator was more efficient than the HDVR, which will be helpful in choosing an influenza virus rescue system, as well as in establishing other RNA virus rescue systems.

引用

页码：1151 / 1156

页数：6

共 19 条

[11] Virus-inducible reporter genes as a tool for detecting and quantifying influenza A virus replication [J].