Human serum proteins preseparated by electrophoresis or chromatography followed by tandem mass spectrometry

被引：70

作者：

Marshall, J ^{[1
]}

Jankowski, A ^{[1
]}

Furesz, S ^{[1
]}

Kireeva, I ^{[1
]}

Barker, L ^{[1
]}

Dombrovsky, M ^{[1
]}

Zhu, WM ^{[1
]}

Jacks, K ^{[1
]}

Ingratta, L ^{[1
]}

Bruin, J ^{[1
]}

Kristensen, E ^{[1
]}

Zhang, RL ^{[1
]}

Stanton, E ^{[1
]}

Takahashi, M ^{[1
]}

Jackowski, G ^{[1
]}

机构：

[1] Synx Pharma, Toronto, ON M9W 1E7, Canada

来源：

JOURNAL OF PROTEOME RESEARCH | 2004年 / 3卷 / 03期

关键词：

human; serum; protein; proteome; electrophoresis; chromatography; tandem; mass spectrometer;

D O I：

10.1021/pr034039p

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Electrophoretic and chromatographic sample preparations were compared and together detected the presence of some 600 types of protein products in human serum. Proteins from crude serum preseparated by ionic electrophoresis, chromatography, or a combination of both were analyzed. Proteins were digested with trypsin or chymotrypsin. Naturally occurring peptides were also collected by reversed-phase chromatography. The resulting peptides were identified by tandem mass spectrometry. The peptides were either desorbed by a laser from a metal chip into a quadrupole-time-of-flight mass spectrometer or ionized as an electro-spray from reversed-phase chromatography via a metal needle under voltage into an ion-trap mass spectrometer. All of the commonly known proteins associated with serum were detected, and the two mass spectrometers agreed on the identity of abundant serum proteins. Preseparation of serum proteins prior to digestion markedly enhanced the capacity to detect un-common proteins from blood. Electrophoretic- and chromatography-based experiments were found to be complementary. Many novel cellular proteins not previously associated with serum were recorded.

引用

页码：364 / 382

页数：19

共 76 条

[71] Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry [J].