Validation and application of an assay for the determination of mevalonic acid in human plasma by liquid chromatography tandem mass spectrometry

被引：15

作者：

Abrar, M ^{[1
]}

Martin, PD ^{[1
]}

机构：

[1] AstraZeneca, Macclesfield SK10 4TG, Cheshire, England

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 773卷 / 02期

关键词：

mevalonic acid;

D O I：

10.1016/S1570-0232(02)00131-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The validation of a method for the determination of mevalonic acid (MVA; after conversion to the lactone, MVAL) in human plasma, using high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS-MS), is reported. MVAL and deuterated internal standard were extracted from human plasma samples using automated solid-phase extraction. Analysis was conducted by column-switching, reversed-phase LC-MS-MS, using two hyper-cross-linked styrene-divinylbenzene copolymer sorbent reversed-phase columns. An assay range of 0.2-35 ng/ml and a lower limit of quantitation (LLOQ) of 0.2 ng/ml were achieved with acceptable accuracy and precision. MVA was stable in plasma under a variety of storage conditions. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：103 / 111

页数：9

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