SIMPLE AND SENSITIVE QUANTITATION METHOD FOR MEVALONIC ACID IN PLASMA USING GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：5

作者：

ISHIHAMA, Y ^{[1
]}

MANO, N ^{[1
]}

ODA, Y ^{[1
]}

HIYOSHI, H ^{[1
]}

IKUTA, H ^{[1
]}

YOSHIDA, Y ^{[1
]}

机构：

[1] EISAI & CO LTD,DEPT CARDIO VASC ATHEROSCLEROSIS & THROMBOSIS RES,TSUKUBA RES LABS,TSUKUBA,IBARAKI 30026,JAPAN

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1994年 / 8卷 / 05期

关键词：

D O I：

10.1002/rcm.1290080507

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The combination of gas chromatography and mass spectrometry using a polar capillary column and isobutane chemical ionization made it possible to determine mevalonic acid (MVA) as the lactone at subnanogram levels using 1 mL of plasma. The pretreatment procedure consisted of only three steps, namely lactonization, washing with chloroform and liquid/liquid extraction. This simple, rapid and sensitive method, having good precision and accuracy, is useful for evaluating the change of plasma MVA, which is well correlated with whole-body cholesterol biosynthesis.

引用

页码：377 / 380

页数：4

共 7 条

[1]

DELPUPPO M, 1989, BIOMED ENVIRON MASS, V13, P174

[2] OXIDATION BY SILVER CARBONATE ON CELITE .13. PREPARATION OF LACTONES [J].

FETIZON, M ;

GOLFIER, M ;

LOUIS, JM .

TETRAHEDRON, 1975, 31 (02) :171-176

[3] AN IMPROVED CHEMICAL IONIZATION ASSAY FOR MEVALONIC ACID [J].

HOFFMANN, GF ;

SWEETMAN, L .

BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, 1990, 19 (08) :517-519

[4] MEVALONIC ACID IN HUMAN-PLASMA - RELATIONSHIP OF CONCENTRATION AND CIRCADIAN-RHYTHM TO CHOLESTEROL-SYNTHESIS RATES IN MAN [J].

PARKER, TS ;

MCNAMARA, DJ ;

BROWN, C ;

GARRIGAN, O ;

KOLB, R ;

BATWIN, H ;

AHRENS, EH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (09) :3037-3041

[5]

POPJAK G, 1979, J LIPID RES, V20, P716

[6]

SCOPPOLA A, 1991, J LIPID RES, V32, P1057

[7] SIMULTANEOUS DETERMINATION OF MEVALONATE AND 7-ALPHA-HYDROXYCHOLESTEROL IN HUMAN PLASMA BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY AS INDEXES OF CHOLESTEROL AND BILE-ACID BIOSYNTHESIS [J].

YOSHIDA, T ;

HONDA, A ;

TANAKA, N ;

MATSUZAKI, Y ;

HE, B ;

OSUGA, T ;

KOBAYASHI, N ;

OZAWA, K ;

MIYAZAKI, H .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1993, 613 (02) :185-193

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