A reliable PCR amplification method for microdissected tumor cells obtained from paraffin-embedded tissue

被引：14

作者：

Akalu, A

Reichardt, JKV

机构：

[1] Univ So Calif, Sch Med, Inst Med Genet, Los Angeles, CA 90033 USA

[2] Univ So Calif, Sch Med, Dept Biochem & Mol Biol, Los Angeles, CA 90033 USA

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1999年 / 15卷 / 06期

关键词：

PCR; tumor cells; paraffin-embedded tissue;

D O I：

10.1016/S1050-3862(99)00050-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a reliable method for PCR (polymerase chain reaction) amplification of genomic DNA from PET. This method uses DNA extraction with the QIAquick kit and amplification with AmpliTaq Gold. Amplification of up to 959 bp from PET was achieved with this combination which exceeds the current reported upper limit of 800 bp. In summary, the gradual activation of the AmpliTaq Gold during thermal cycling allows both for higher-fidelity and higher-throughput PCR amplification from PET. The use of the QIAquick kit for DNA purification of PET is sensitive, reproducible and suitable for management of a high number of samples. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：229 / 233

页数：5